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Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability
Fluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effect...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249074/ https://www.ncbi.nlm.nih.gov/pubmed/32344920 http://dx.doi.org/10.3390/molecules25092013 |
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author | Jarzębski, Maciej Siejak, Przemysław Przeor, Monika Gapiński, Jacek Woźniak, Anna Baranowska, Hanna Maria Pawlicz, Jarosław Baryła-Pankiewicz, Elżbieta Szwajca, Anna |
author_facet | Jarzębski, Maciej Siejak, Przemysław Przeor, Monika Gapiński, Jacek Woźniak, Anna Baranowska, Hanna Maria Pawlicz, Jarosław Baryła-Pankiewicz, Elżbieta Szwajca, Anna |
author_sort | Jarzębski, Maciej |
collection | PubMed |
description | Fluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effectively protected the dye against bleaching. HFBMA nanoparticle (NP) stability was confirmed after seven years of storage. Only slight differences were found in the polydispersity index (pdi) from 0.002 to 0.010. Particle size measurements were carried out using dynamic light scattering (DLS), nanoparticle tracking (NTA), and fluorescence correlation spectroscopy (FCS). The hydrodynamic diameter evaluated by different methods were in good agreement, respectively: 184–550 nm, 218–579 nm, and 236–508 nm. Particle and core morphology was estimated by using scanning and transmission electron microscopy (SEM and TEM). The ability to recognize particles in 3D as a reference sample in biological media has been confirmed by epifluorescence optical microscopy, confocal laser scanning microscopy, and super-resolution confocal microscopy (STED). |
format | Online Article Text |
id | pubmed-7249074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72490742020-06-10 Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability Jarzębski, Maciej Siejak, Przemysław Przeor, Monika Gapiński, Jacek Woźniak, Anna Baranowska, Hanna Maria Pawlicz, Jarosław Baryła-Pankiewicz, Elżbieta Szwajca, Anna Molecules Article Fluorescent submicron particles of fluorinated methacrylate (HFMBA) with long-term stability have been synthesized and characterized with regard to their potential applications. Rhodamine B (RBITC) isothiocyanate was used as the fluorescent component. The core–shell structure of the particles effectively protected the dye against bleaching. HFBMA nanoparticle (NP) stability was confirmed after seven years of storage. Only slight differences were found in the polydispersity index (pdi) from 0.002 to 0.010. Particle size measurements were carried out using dynamic light scattering (DLS), nanoparticle tracking (NTA), and fluorescence correlation spectroscopy (FCS). The hydrodynamic diameter evaluated by different methods were in good agreement, respectively: 184–550 nm, 218–579 nm, and 236–508 nm. Particle and core morphology was estimated by using scanning and transmission electron microscopy (SEM and TEM). The ability to recognize particles in 3D as a reference sample in biological media has been confirmed by epifluorescence optical microscopy, confocal laser scanning microscopy, and super-resolution confocal microscopy (STED). MDPI 2020-04-25 /pmc/articles/PMC7249074/ /pubmed/32344920 http://dx.doi.org/10.3390/molecules25092013 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jarzębski, Maciej Siejak, Przemysław Przeor, Monika Gapiński, Jacek Woźniak, Anna Baranowska, Hanna Maria Pawlicz, Jarosław Baryła-Pankiewicz, Elżbieta Szwajca, Anna Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability |
title | Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability |
title_full | Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability |
title_fullStr | Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability |
title_full_unstemmed | Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability |
title_short | Fluorescent Submicron-Sized Poly(heptafluoro-n-butyl methacrylate) Particles with Long-Term Stability |
title_sort | fluorescent submicron-sized poly(heptafluoro-n-butyl methacrylate) particles with long-term stability |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249074/ https://www.ncbi.nlm.nih.gov/pubmed/32344920 http://dx.doi.org/10.3390/molecules25092013 |
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