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Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus

The early diagnostics of hepatitis C virus (HCV) infections is currently one of the most highly demanded medical tasks. This study is devoted to the development of biochips (microarrays) that can be applied for the detection of HCV. The analytical platforms of suggested devices were based on macropo...

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Autores principales: Antipchik, Mariia, Polyakov, Dmitry, Sinitsyna, Ekaterina, Dzhuzha, Apollinariia, Shavlovsky, Mikhail, Korzhikova-Vlakh, Evgenia, Tennikova, Tatiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249126/
https://www.ncbi.nlm.nih.gov/pubmed/32397590
http://dx.doi.org/10.3390/s20092719
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author Antipchik, Mariia
Polyakov, Dmitry
Sinitsyna, Ekaterina
Dzhuzha, Apollinariia
Shavlovsky, Mikhail
Korzhikova-Vlakh, Evgenia
Tennikova, Tatiana
author_facet Antipchik, Mariia
Polyakov, Dmitry
Sinitsyna, Ekaterina
Dzhuzha, Apollinariia
Shavlovsky, Mikhail
Korzhikova-Vlakh, Evgenia
Tennikova, Tatiana
author_sort Antipchik, Mariia
collection PubMed
description The early diagnostics of hepatitis C virus (HCV) infections is currently one of the most highly demanded medical tasks. This study is devoted to the development of biochips (microarrays) that can be applied for the detection of HCV. The analytical platforms of suggested devices were based on macroporous poly(glycidyl methacrylate-co-di(ethylene glycol) dimethacrylate) monolithic material. The biochips were obtained by the covalent immobilization of specific probes spotted onto the surface of macroporous monolithic platforms. Using the developed biochips, different variants of bioassay were investigated. This study was carried out using hepatitis C virus-mimetic particles (VMPs) representing polymer nanoparticles with a size close to HCV and bearing surface virus antigen (E2 protein). At the first step, the main parameters of bioassay were optimized. Additionally, the dissociation constants were calculated for the pairs “ligand–receptor” and “antigen–antibody” formed at the surface of biochips. As a result of this study, the analysis of VMPs in model buffer solution and human blood plasma was carried out in a format of direct and “sandwich” approaches. It was found that bioassay efficacy appeared to be similar for both the model medium and real biological fluid. Finally, limit of detection (LOD), limit of quantification (LOQ), spot-to-spot and biochip-to-biochip reproducibility for the developed systems were evaluated.
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spelling pubmed-72491262020-06-10 Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus Antipchik, Mariia Polyakov, Dmitry Sinitsyna, Ekaterina Dzhuzha, Apollinariia Shavlovsky, Mikhail Korzhikova-Vlakh, Evgenia Tennikova, Tatiana Sensors (Basel) Article The early diagnostics of hepatitis C virus (HCV) infections is currently one of the most highly demanded medical tasks. This study is devoted to the development of biochips (microarrays) that can be applied for the detection of HCV. The analytical platforms of suggested devices were based on macroporous poly(glycidyl methacrylate-co-di(ethylene glycol) dimethacrylate) monolithic material. The biochips were obtained by the covalent immobilization of specific probes spotted onto the surface of macroporous monolithic platforms. Using the developed biochips, different variants of bioassay were investigated. This study was carried out using hepatitis C virus-mimetic particles (VMPs) representing polymer nanoparticles with a size close to HCV and bearing surface virus antigen (E2 protein). At the first step, the main parameters of bioassay were optimized. Additionally, the dissociation constants were calculated for the pairs “ligand–receptor” and “antigen–antibody” formed at the surface of biochips. As a result of this study, the analysis of VMPs in model buffer solution and human blood plasma was carried out in a format of direct and “sandwich” approaches. It was found that bioassay efficacy appeared to be similar for both the model medium and real biological fluid. Finally, limit of detection (LOD), limit of quantification (LOQ), spot-to-spot and biochip-to-biochip reproducibility for the developed systems were evaluated. MDPI 2020-05-10 /pmc/articles/PMC7249126/ /pubmed/32397590 http://dx.doi.org/10.3390/s20092719 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Antipchik, Mariia
Polyakov, Dmitry
Sinitsyna, Ekaterina
Dzhuzha, Apollinariia
Shavlovsky, Mikhail
Korzhikova-Vlakh, Evgenia
Tennikova, Tatiana
Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus
title Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus
title_full Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus
title_fullStr Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus
title_full_unstemmed Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus
title_short Towards the Development of a 3-D Biochip for the Detection of Hepatitis C Virus
title_sort towards the development of a 3-d biochip for the detection of hepatitis c virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249126/
https://www.ncbi.nlm.nih.gov/pubmed/32397590
http://dx.doi.org/10.3390/s20092719
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