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Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility

BACKGROUND: Sperm hyperactive motility has previously been shown to influence litter size in pigs, but little is known about the underlying biological mechanisms. The aim of this study was to use RNA sequencing to investigate gene expression differences in testis tissue from Landrace and Duroc boars...

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Autores principales: van Son, Maren, Tremoen, Nina Hårdnes, Gaustad, Ann Helen, Våge, Dag Inge, Zeremichael, Teklu Tewoldebrhan, Myromslien, Frøydis Deinboll, Grindflek, Eli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249385/
https://www.ncbi.nlm.nih.gov/pubmed/32456687
http://dx.doi.org/10.1186/s12917-020-02373-9
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author van Son, Maren
Tremoen, Nina Hårdnes
Gaustad, Ann Helen
Våge, Dag Inge
Zeremichael, Teklu Tewoldebrhan
Myromslien, Frøydis Deinboll
Grindflek, Eli
author_facet van Son, Maren
Tremoen, Nina Hårdnes
Gaustad, Ann Helen
Våge, Dag Inge
Zeremichael, Teklu Tewoldebrhan
Myromslien, Frøydis Deinboll
Grindflek, Eli
author_sort van Son, Maren
collection PubMed
description BACKGROUND: Sperm hyperactive motility has previously been shown to influence litter size in pigs, but little is known about the underlying biological mechanisms. The aim of this study was to use RNA sequencing to investigate gene expression differences in testis tissue from Landrace and Duroc boars with high and low levels of sperm hyperactive motility. Boars with divergent phenotypes were selected based on their sperm hyperactivity values at the day of ejaculation (day 0) (contrasts (i) and (ii) for Landrace and Duroc, respectively) and on their change in hyperactivity between day 0 and after 96 h liquid storage at 18 °C (contrast (iii)). RESULTS: RNA sequencing was used to measure gene expression in testis. In Landrace boars, 3219 genes were differentially expressed for contrast (i), whereas 102 genes were differentially expressed for contrast (iii). Forty-one differentially expressed genes were identified in both contrasts, suggesting a functional role of these genes in hyperactivity regardless of storage. Zinc finger DNLZ was the most up-regulated gene in contrasts (i) and (iii), whereas the most significant differentially expressed gene for the two contrasts were ADP ribosylation factor ARFGAP1 and solute carrier SLC40A1, respectively. For Duroc (contrast (ii)), the clustering of boars based on their gene expression data did not reflect their difference in sperm hyperactivity phenotypes. No results were therefore obtained for this breed. A case-control analysis of variants identified in the Landrace RNA sequencing data showed that SNPs in NEU3, CHRDL2 and HMCN1 might be important for sperm hyperactivity. CONCLUSIONS: Differentially expressed genes were identified in Landrace boars with high and low levels of sperm hyperactivity at the day of ejaculate collection and high and low change in hyperactivity after 96 h of sperm storage. The results point towards important candidate genes, biochemical pathways and sequence variants underlying sperm hyperactivity in pigs.
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spelling pubmed-72493852020-06-04 Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility van Son, Maren Tremoen, Nina Hårdnes Gaustad, Ann Helen Våge, Dag Inge Zeremichael, Teklu Tewoldebrhan Myromslien, Frøydis Deinboll Grindflek, Eli BMC Vet Res Research Article BACKGROUND: Sperm hyperactive motility has previously been shown to influence litter size in pigs, but little is known about the underlying biological mechanisms. The aim of this study was to use RNA sequencing to investigate gene expression differences in testis tissue from Landrace and Duroc boars with high and low levels of sperm hyperactive motility. Boars with divergent phenotypes were selected based on their sperm hyperactivity values at the day of ejaculation (day 0) (contrasts (i) and (ii) for Landrace and Duroc, respectively) and on their change in hyperactivity between day 0 and after 96 h liquid storage at 18 °C (contrast (iii)). RESULTS: RNA sequencing was used to measure gene expression in testis. In Landrace boars, 3219 genes were differentially expressed for contrast (i), whereas 102 genes were differentially expressed for contrast (iii). Forty-one differentially expressed genes were identified in both contrasts, suggesting a functional role of these genes in hyperactivity regardless of storage. Zinc finger DNLZ was the most up-regulated gene in contrasts (i) and (iii), whereas the most significant differentially expressed gene for the two contrasts were ADP ribosylation factor ARFGAP1 and solute carrier SLC40A1, respectively. For Duroc (contrast (ii)), the clustering of boars based on their gene expression data did not reflect their difference in sperm hyperactivity phenotypes. No results were therefore obtained for this breed. A case-control analysis of variants identified in the Landrace RNA sequencing data showed that SNPs in NEU3, CHRDL2 and HMCN1 might be important for sperm hyperactivity. CONCLUSIONS: Differentially expressed genes were identified in Landrace boars with high and low levels of sperm hyperactivity at the day of ejaculate collection and high and low change in hyperactivity after 96 h of sperm storage. The results point towards important candidate genes, biochemical pathways and sequence variants underlying sperm hyperactivity in pigs. BioMed Central 2020-05-26 /pmc/articles/PMC7249385/ /pubmed/32456687 http://dx.doi.org/10.1186/s12917-020-02373-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
van Son, Maren
Tremoen, Nina Hårdnes
Gaustad, Ann Helen
Våge, Dag Inge
Zeremichael, Teklu Tewoldebrhan
Myromslien, Frøydis Deinboll
Grindflek, Eli
Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
title Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
title_full Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
title_fullStr Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
title_full_unstemmed Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
title_short Transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
title_sort transcriptome profiling of porcine testis tissue reveals genes related to sperm hyperactive motility
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249385/
https://www.ncbi.nlm.nih.gov/pubmed/32456687
http://dx.doi.org/10.1186/s12917-020-02373-9
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