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Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages

BACKGROUND: Necrotizing enterocolitis (NEC) is associated with significant morbidity and mortality. Serum biomarkers to aid diagnosis, such as intestinal fatty acid binding protein (IFABP) and calprotectin, are actively being investigated; however, the normative values of these markers among healthy...

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Autores principales: Shores, Darla R., Fundora, Jennifer, Go, Mitzi, Shakeel, Fauzia, Brooks, Sandra, Alaish, Samuel M., Yang, Jun, Sodhi, Chhinder P., Hackam, David J., Everett, Allen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249444/
https://www.ncbi.nlm.nih.gov/pubmed/32456678
http://dx.doi.org/10.1186/s12887-020-02142-5
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author Shores, Darla R.
Fundora, Jennifer
Go, Mitzi
Shakeel, Fauzia
Brooks, Sandra
Alaish, Samuel M.
Yang, Jun
Sodhi, Chhinder P.
Hackam, David J.
Everett, Allen
author_facet Shores, Darla R.
Fundora, Jennifer
Go, Mitzi
Shakeel, Fauzia
Brooks, Sandra
Alaish, Samuel M.
Yang, Jun
Sodhi, Chhinder P.
Hackam, David J.
Everett, Allen
author_sort Shores, Darla R.
collection PubMed
description BACKGROUND: Necrotizing enterocolitis (NEC) is associated with significant morbidity and mortality. Serum biomarkers to aid diagnosis, such as intestinal fatty acid binding protein (IFABP) and calprotectin, are actively being investigated; however, the normative values of these markers among healthy premature and term infants remains unknown. We sought to identify normative values for the serum concentrations of IFABP and calprotectin across gestational (GA) and post-menstrual age. METHODS: We collected serum from infants (24–40 weeks GA) in the first week of life and at multiple time points in a sub-cohort of premature infants (24–29 weeks GA), excluding sepsis or known intestinal disease. IFABP and calprotectin were measured using ELISA. Groups were compared with descriptive statistics and mixed effects linear regression. RESULTS: One hundred twelve infants had specimens in the first week of life, and 19 premature infants had longitudinal specimens. IFABP concentration in the first week of life was low and did not differ across gestational ages. Longitudinally, IFABP increased 4% per day (P < 0.001). Calprotectin concentration in the first week of life was more variable. An inverse relationship between day of life and calprotectin level was found in the longitudinal cohort (P < 0.001). CONCLUSIONS: Serum IFABP and calprotectin fluctuate over time. Infants had low levels of IFABP during the first week of life, independent of gestational age, and levels increased longitudinally in premature infants. Calprotectin levels generally declined over time. Normative data for infants is necessary to establish meaningful cut-off levels for clinical use.
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spelling pubmed-72494442020-06-04 Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages Shores, Darla R. Fundora, Jennifer Go, Mitzi Shakeel, Fauzia Brooks, Sandra Alaish, Samuel M. Yang, Jun Sodhi, Chhinder P. Hackam, David J. Everett, Allen BMC Pediatr Research Article BACKGROUND: Necrotizing enterocolitis (NEC) is associated with significant morbidity and mortality. Serum biomarkers to aid diagnosis, such as intestinal fatty acid binding protein (IFABP) and calprotectin, are actively being investigated; however, the normative values of these markers among healthy premature and term infants remains unknown. We sought to identify normative values for the serum concentrations of IFABP and calprotectin across gestational (GA) and post-menstrual age. METHODS: We collected serum from infants (24–40 weeks GA) in the first week of life and at multiple time points in a sub-cohort of premature infants (24–29 weeks GA), excluding sepsis or known intestinal disease. IFABP and calprotectin were measured using ELISA. Groups were compared with descriptive statistics and mixed effects linear regression. RESULTS: One hundred twelve infants had specimens in the first week of life, and 19 premature infants had longitudinal specimens. IFABP concentration in the first week of life was low and did not differ across gestational ages. Longitudinally, IFABP increased 4% per day (P < 0.001). Calprotectin concentration in the first week of life was more variable. An inverse relationship between day of life and calprotectin level was found in the longitudinal cohort (P < 0.001). CONCLUSIONS: Serum IFABP and calprotectin fluctuate over time. Infants had low levels of IFABP during the first week of life, independent of gestational age, and levels increased longitudinally in premature infants. Calprotectin levels generally declined over time. Normative data for infants is necessary to establish meaningful cut-off levels for clinical use. BioMed Central 2020-05-26 /pmc/articles/PMC7249444/ /pubmed/32456678 http://dx.doi.org/10.1186/s12887-020-02142-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Shores, Darla R.
Fundora, Jennifer
Go, Mitzi
Shakeel, Fauzia
Brooks, Sandra
Alaish, Samuel M.
Yang, Jun
Sodhi, Chhinder P.
Hackam, David J.
Everett, Allen
Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
title Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
title_full Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
title_fullStr Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
title_full_unstemmed Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
title_short Normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
title_sort normative values for circulating intestinal fatty acid binding protein and calprotectin across gestational ages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7249444/
https://www.ncbi.nlm.nih.gov/pubmed/32456678
http://dx.doi.org/10.1186/s12887-020-02142-5
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