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Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes

We report the possibility of a time-resolved bacterial live/dead dynamics observation with the use of plasmonic nanospikes. Sharp nanospikes, fabricated on a 500-nm thick gold film by laser ablation with the use of 1030-nm femtosecond pulses, were tested as potential elements for antibacterial surfa...

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Autores principales: Saraeva, Irina, Kudryashov, Sergey I., Danilov, Pavel, Busleev, Nikolay, Tolordava, Eteri R., Rudenko, Andrey A., Zayarny, Dmitriy, Ionin, Andrey, Romanova, Yulia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250023/
https://www.ncbi.nlm.nih.gov/pubmed/32349257
http://dx.doi.org/10.3390/s20092466
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author Saraeva, Irina
Kudryashov, Sergey I.
Danilov, Pavel
Busleev, Nikolay
Tolordava, Eteri R.
Rudenko, Andrey A.
Zayarny, Dmitriy
Ionin, Andrey
Romanova, Yulia M.
author_facet Saraeva, Irina
Kudryashov, Sergey I.
Danilov, Pavel
Busleev, Nikolay
Tolordava, Eteri R.
Rudenko, Andrey A.
Zayarny, Dmitriy
Ionin, Andrey
Romanova, Yulia M.
author_sort Saraeva, Irina
collection PubMed
description We report the possibility of a time-resolved bacterial live/dead dynamics observation with the use of plasmonic nanospikes. Sharp nanospikes, fabricated on a 500-nm thick gold film by laser ablation with the use of 1030-nm femtosecond pulses, were tested as potential elements for antibacterial surfaces and plasmonic luminescence sensors. Staphylococcus aureus bacteria were stained by a live/dead viability kit, with the dead microorganisms acquiring the red colour, caused by the penetration of the luminescent dye propidium iodide through the damaged cell membrane. Photoluminescence was pumped by 515-nm femtosecond laser pulses with linear (Gaussian beam), circular, azimuthal and radial (Laguerre–Gaussian beam) polarizations, exciting the transverse plasmon resonance of the nanospikes and their apex lightning-rod near-field. According to the numerical electrodynamic modeling, the observed strong increase in the photoluminescence yield for radial polarization, while slightly lower for circular and azimuthal polarizations, compared with the low luminescence intensities for the linear laser polarization, was related to their different laser–nanospike coupling efficiencies.
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spelling pubmed-72500232020-06-10 Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes Saraeva, Irina Kudryashov, Sergey I. Danilov, Pavel Busleev, Nikolay Tolordava, Eteri R. Rudenko, Andrey A. Zayarny, Dmitriy Ionin, Andrey Romanova, Yulia M. Sensors (Basel) Article We report the possibility of a time-resolved bacterial live/dead dynamics observation with the use of plasmonic nanospikes. Sharp nanospikes, fabricated on a 500-nm thick gold film by laser ablation with the use of 1030-nm femtosecond pulses, were tested as potential elements for antibacterial surfaces and plasmonic luminescence sensors. Staphylococcus aureus bacteria were stained by a live/dead viability kit, with the dead microorganisms acquiring the red colour, caused by the penetration of the luminescent dye propidium iodide through the damaged cell membrane. Photoluminescence was pumped by 515-nm femtosecond laser pulses with linear (Gaussian beam), circular, azimuthal and radial (Laguerre–Gaussian beam) polarizations, exciting the transverse plasmon resonance of the nanospikes and their apex lightning-rod near-field. According to the numerical electrodynamic modeling, the observed strong increase in the photoluminescence yield for radial polarization, while slightly lower for circular and azimuthal polarizations, compared with the low luminescence intensities for the linear laser polarization, was related to their different laser–nanospike coupling efficiencies. MDPI 2020-04-27 /pmc/articles/PMC7250023/ /pubmed/32349257 http://dx.doi.org/10.3390/s20092466 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saraeva, Irina
Kudryashov, Sergey I.
Danilov, Pavel
Busleev, Nikolay
Tolordava, Eteri R.
Rudenko, Andrey A.
Zayarny, Dmitriy
Ionin, Andrey
Romanova, Yulia M.
Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes
title Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes
title_full Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes
title_fullStr Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes
title_full_unstemmed Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes
title_short Polarization-Sensitive Surface-Enhanced In Situ Photoluminescence Spectroscopy of S. aureus Bacteria on Gold Nanospikes
title_sort polarization-sensitive surface-enhanced in situ photoluminescence spectroscopy of s. aureus bacteria on gold nanospikes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250023/
https://www.ncbi.nlm.nih.gov/pubmed/32349257
http://dx.doi.org/10.3390/s20092466
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