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Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Her...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250415/ https://www.ncbi.nlm.nih.gov/pubmed/32453735 http://dx.doi.org/10.1371/journal.pone.0233394 |
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author | Alendar, Andrej Lambooij, Jan-Paul Bhaskaran, Rajith Lancini, Cesare Song, Ji-Ying van Vugt, Huub Snoek, Margriet Berns, Anton |
author_facet | Alendar, Andrej Lambooij, Jan-Paul Bhaskaran, Rajith Lancini, Cesare Song, Ji-Ying van Vugt, Huub Snoek, Margriet Berns, Anton |
author_sort | Alendar, Andrej |
collection | PubMed |
description | Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9(–/–)animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development. |
format | Online Article Text |
id | pubmed-7250415 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-72504152020-06-08 Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development Alendar, Andrej Lambooij, Jan-Paul Bhaskaran, Rajith Lancini, Cesare Song, Ji-Ying van Vugt, Huub Snoek, Margriet Berns, Anton PLoS One Research Article Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9(–/–)animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development. Public Library of Science 2020-05-26 /pmc/articles/PMC7250415/ /pubmed/32453735 http://dx.doi.org/10.1371/journal.pone.0233394 Text en © 2020 Alendar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Alendar, Andrej Lambooij, Jan-Paul Bhaskaran, Rajith Lancini, Cesare Song, Ji-Ying van Vugt, Huub Snoek, Margriet Berns, Anton Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development |
title | Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development |
title_full | Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development |
title_fullStr | Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development |
title_full_unstemmed | Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development |
title_short | Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development |
title_sort | gene expression regulation by the chromodomain helicase dna-binding protein 9 (chd9) chromatin remodeler is dispensable for murine development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250415/ https://www.ncbi.nlm.nih.gov/pubmed/32453735 http://dx.doi.org/10.1371/journal.pone.0233394 |
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