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Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development

Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Her...

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Autores principales: Alendar, Andrej, Lambooij, Jan-Paul, Bhaskaran, Rajith, Lancini, Cesare, Song, Ji-Ying, van Vugt, Huub, Snoek, Margriet, Berns, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250415/
https://www.ncbi.nlm.nih.gov/pubmed/32453735
http://dx.doi.org/10.1371/journal.pone.0233394
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author Alendar, Andrej
Lambooij, Jan-Paul
Bhaskaran, Rajith
Lancini, Cesare
Song, Ji-Ying
van Vugt, Huub
Snoek, Margriet
Berns, Anton
author_facet Alendar, Andrej
Lambooij, Jan-Paul
Bhaskaran, Rajith
Lancini, Cesare
Song, Ji-Ying
van Vugt, Huub
Snoek, Margriet
Berns, Anton
author_sort Alendar, Andrej
collection PubMed
description Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9(–/–)animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development.
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spelling pubmed-72504152020-06-08 Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development Alendar, Andrej Lambooij, Jan-Paul Bhaskaran, Rajith Lancini, Cesare Song, Ji-Ying van Vugt, Huub Snoek, Margriet Berns, Anton PLoS One Research Article Chromodomain helicase DNA-binding (CHD) chromatin remodelers regulate transcription and DNA repair. They govern cell-fate decisions during embryonic development and are often deregulated in human pathologies. Chd1-8 show upon germline disruption pronounced, often developmental lethal phenotypes. Here we show that contrary to Chd1-8 disruption, Chd9(–/–)animals are viable, fertile and display no developmental defects or disease predisposition. Germline deletion of Chd9 only moderately affects gene expression in tissues and derived cells, whereas acute depletion in human cancer cells elicits more robust changes suggesting that CHD9 is a highly context-dependent chromatin regulator that, surprisingly, is dispensable for mouse development. Public Library of Science 2020-05-26 /pmc/articles/PMC7250415/ /pubmed/32453735 http://dx.doi.org/10.1371/journal.pone.0233394 Text en © 2020 Alendar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Alendar, Andrej
Lambooij, Jan-Paul
Bhaskaran, Rajith
Lancini, Cesare
Song, Ji-Ying
van Vugt, Huub
Snoek, Margriet
Berns, Anton
Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
title Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
title_full Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
title_fullStr Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
title_full_unstemmed Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
title_short Gene expression regulation by the Chromodomain helicase DNA-binding protein 9 (CHD9) chromatin remodeler is dispensable for murine development
title_sort gene expression regulation by the chromodomain helicase dna-binding protein 9 (chd9) chromatin remodeler is dispensable for murine development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250415/
https://www.ncbi.nlm.nih.gov/pubmed/32453735
http://dx.doi.org/10.1371/journal.pone.0233394
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