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Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants

Glyoxalase pathway is the major pathway of methylglyoxal detoxification and is ubiquitously present in all organisms ranging from prokaryotes to eukaryotes. Glyoxalase I (GLYI) and Glyoxalase II (GLYII), the two core enzymes of this pathway work together to neutralize methylglyoxal (MG), a dicarbony...

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Autores principales: Batth, Rituraj, Jain, Muskan, Kumar, Ashish, Nagar, Preeti, Kumari, Sumita, Mustafiz, Ananda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250436/
https://www.ncbi.nlm.nih.gov/pubmed/32453778
http://dx.doi.org/10.1371/journal.pone.0233493
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author Batth, Rituraj
Jain, Muskan
Kumar, Ashish
Nagar, Preeti
Kumari, Sumita
Mustafiz, Ananda
author_facet Batth, Rituraj
Jain, Muskan
Kumar, Ashish
Nagar, Preeti
Kumari, Sumita
Mustafiz, Ananda
author_sort Batth, Rituraj
collection PubMed
description Glyoxalase pathway is the major pathway of methylglyoxal detoxification and is ubiquitously present in all organisms ranging from prokaryotes to eukaryotes. Glyoxalase I (GLYI) and Glyoxalase II (GLYII), the two core enzymes of this pathway work together to neutralize methylglyoxal (MG), a dicarbonyl molecule with detrimental cytotoxicity at higher concentrations. The first step towards the detoxification of MG is catalyzed by GLYI, a metalloenzyme that requires divalent metal ions (either Zn(2+) as seen in eukaryotes or Ni(2+) as in prokaryotes). However, both Zn(2+) and Ni(2+) dependent GLYIs have been shown to co-exist in a higher eukaryote i.e. Arabidopsis thaliana. In the present study, we determine the role of both Zn(2+) dependent (AtGLYI2) and Ni(2+) dependent (AtGLYI3, AtGLYI6) GLYIs from Arabidopsis in salinity stress tolerance. AtGLYI2 overexpressing Arabidopsis plants showed better growth rate while maintaining lower levels of MG under high saline conditions. They were taller with more number of silique formation with respect to their Ni(2+) dependent counterparts. Further, lack in germination of Arabidopsis AtGLYI2 mutants in presence of exogenous MG indicates the direct involvement of Zn(2+) dependent GLYI in MG detoxification, suggesting Zn(2+) dependent GLYI as the main enzyme responsible for MG detoxification and salinity stress tolerance.
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spelling pubmed-72504362020-06-08 Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants Batth, Rituraj Jain, Muskan Kumar, Ashish Nagar, Preeti Kumari, Sumita Mustafiz, Ananda PLoS One Research Article Glyoxalase pathway is the major pathway of methylglyoxal detoxification and is ubiquitously present in all organisms ranging from prokaryotes to eukaryotes. Glyoxalase I (GLYI) and Glyoxalase II (GLYII), the two core enzymes of this pathway work together to neutralize methylglyoxal (MG), a dicarbonyl molecule with detrimental cytotoxicity at higher concentrations. The first step towards the detoxification of MG is catalyzed by GLYI, a metalloenzyme that requires divalent metal ions (either Zn(2+) as seen in eukaryotes or Ni(2+) as in prokaryotes). However, both Zn(2+) and Ni(2+) dependent GLYIs have been shown to co-exist in a higher eukaryote i.e. Arabidopsis thaliana. In the present study, we determine the role of both Zn(2+) dependent (AtGLYI2) and Ni(2+) dependent (AtGLYI3, AtGLYI6) GLYIs from Arabidopsis in salinity stress tolerance. AtGLYI2 overexpressing Arabidopsis plants showed better growth rate while maintaining lower levels of MG under high saline conditions. They were taller with more number of silique formation with respect to their Ni(2+) dependent counterparts. Further, lack in germination of Arabidopsis AtGLYI2 mutants in presence of exogenous MG indicates the direct involvement of Zn(2+) dependent GLYI in MG detoxification, suggesting Zn(2+) dependent GLYI as the main enzyme responsible for MG detoxification and salinity stress tolerance. Public Library of Science 2020-05-26 /pmc/articles/PMC7250436/ /pubmed/32453778 http://dx.doi.org/10.1371/journal.pone.0233493 Text en © 2020 Batth et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Batth, Rituraj
Jain, Muskan
Kumar, Ashish
Nagar, Preeti
Kumari, Sumita
Mustafiz, Ananda
Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
title Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
title_full Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
title_fullStr Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
title_full_unstemmed Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
title_short Zn(2+) dependent glyoxalase I plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
title_sort zn(2+) dependent glyoxalase i plays the major role in methylglyoxal detoxification and salinity stress tolerance in plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250436/
https://www.ncbi.nlm.nih.gov/pubmed/32453778
http://dx.doi.org/10.1371/journal.pone.0233493
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