Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568

Hot-band absorption and anti-Stokes emission properties of an organic fluorescent dye, Alexa Fluor 568, were characterized and compared with those of Rhodamine 101. The comparison of the properties (e.g., quantum efficiency, spectral distribution, thermal properties, and fluorescence lifetime) betwe...

Descripción completa

Detalles Bibliográficos
Autores principales: Gajdos, Tamás, Hopp, Béla, Erdélyi, Miklós
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250809/
https://www.ncbi.nlm.nih.gov/pubmed/32112289
http://dx.doi.org/10.1007/s10895-020-02496-0
_version_ 1783538828587302912
author Gajdos, Tamás
Hopp, Béla
Erdélyi, Miklós
author_facet Gajdos, Tamás
Hopp, Béla
Erdélyi, Miklós
author_sort Gajdos, Tamás
collection PubMed
description Hot-band absorption and anti-Stokes emission properties of an organic fluorescent dye, Alexa Fluor 568, were characterized and compared with those of Rhodamine 101. The comparison of the properties (e.g., quantum efficiency, spectral distribution, thermal properties, and fluorescence lifetime) between the two dyes confirms that both dyes undergo the same process when excited in the red spectral region. Possible undesirable crosstalk effects and applications in dSTORM microscopy were demonstrated and discussed.
format Online
Article
Text
id pubmed-7250809
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-72508092020-06-04 Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568 Gajdos, Tamás Hopp, Béla Erdélyi, Miklós J Fluoresc Original Article Hot-band absorption and anti-Stokes emission properties of an organic fluorescent dye, Alexa Fluor 568, were characterized and compared with those of Rhodamine 101. The comparison of the properties (e.g., quantum efficiency, spectral distribution, thermal properties, and fluorescence lifetime) between the two dyes confirms that both dyes undergo the same process when excited in the red spectral region. Possible undesirable crosstalk effects and applications in dSTORM microscopy were demonstrated and discussed. Springer US 2020-02-29 2020 /pmc/articles/PMC7250809/ /pubmed/32112289 http://dx.doi.org/10.1007/s10895-020-02496-0 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Gajdos, Tamás
Hopp, Béla
Erdélyi, Miklós
Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568
title Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568
title_full Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568
title_fullStr Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568
title_full_unstemmed Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568
title_short Hot-Band Anti-Stokes Fluorescence Properties of Alexa Fluor 568
title_sort hot-band anti-stokes fluorescence properties of alexa fluor 568
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250809/
https://www.ncbi.nlm.nih.gov/pubmed/32112289
http://dx.doi.org/10.1007/s10895-020-02496-0
work_keys_str_mv AT gajdostamas hotbandantistokesfluorescencepropertiesofalexafluor568
AT hoppbela hotbandantistokesfluorescencepropertiesofalexafluor568
AT erdelyimiklos hotbandantistokesfluorescencepropertiesofalexafluor568

Ejemplares similares