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Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients

Helicobacter pylori (HP) infection is an established causative agent for gastric cancer. Although the oral cavity is a part of the gastrointestinal system, the presence and possible causative role of HP in oral squamous cell carcinoma (OSCC) is a subject of controversy. Therefore, the current study...

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Autores principales: Pandey, Sushma, Follin-Arbelet, Benoit, Pun, Chin Bahadur, Gautam, Dej K., Johannessen, Anne Christine, Petersen, Fernanda Cristina, Costea, Daniela Elena, Sapkota, Dipak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250879/
https://www.ncbi.nlm.nih.gov/pubmed/32457404
http://dx.doi.org/10.1038/s41598-020-65694-7
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author Pandey, Sushma
Follin-Arbelet, Benoit
Pun, Chin Bahadur
Gautam, Dej K.
Johannessen, Anne Christine
Petersen, Fernanda Cristina
Costea, Daniela Elena
Sapkota, Dipak
author_facet Pandey, Sushma
Follin-Arbelet, Benoit
Pun, Chin Bahadur
Gautam, Dej K.
Johannessen, Anne Christine
Petersen, Fernanda Cristina
Costea, Daniela Elena
Sapkota, Dipak
author_sort Pandey, Sushma
collection PubMed
description Helicobacter pylori (HP) infection is an established causative agent for gastric cancer. Although the oral cavity is a part of the gastrointestinal system, the presence and possible causative role of HP in oral squamous cell carcinoma (OSCC) is a subject of controversy. Therefore, the current study aimed to investigate HP infection in two cohorts of OSCC patients with different demographic characteristics, lifestyles and habitual risk factors. A total of 242 formalin-fixed paraffin-embedded OSCC specimens from two different patient cohorts (Norway, n = 171 and Nepal, n = 71) were used to examine HP using immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR). Two different HP specific genes (23S rRNA and ureA) were used for TaqMan-based qPCR, and for subsequent verification using HP specific RIDAGENE HP kit and SYBR Green based qPCR. All of the OSCC specimens from both cohorts were found to be negative for HP infection with IHC and qPCR, although the positive control specimens tested positive. Our findings suggest that HP is absent in the examined OSCC cohorts, irrespective of race, lifestyle and habitual risk factors. This indicates that, in contrast to gastric cancer, HP is an unlikely contributing factor for OSCC pathogenesis.
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spelling pubmed-72508792020-06-04 Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients Pandey, Sushma Follin-Arbelet, Benoit Pun, Chin Bahadur Gautam, Dej K. Johannessen, Anne Christine Petersen, Fernanda Cristina Costea, Daniela Elena Sapkota, Dipak Sci Rep Article Helicobacter pylori (HP) infection is an established causative agent for gastric cancer. Although the oral cavity is a part of the gastrointestinal system, the presence and possible causative role of HP in oral squamous cell carcinoma (OSCC) is a subject of controversy. Therefore, the current study aimed to investigate HP infection in two cohorts of OSCC patients with different demographic characteristics, lifestyles and habitual risk factors. A total of 242 formalin-fixed paraffin-embedded OSCC specimens from two different patient cohorts (Norway, n = 171 and Nepal, n = 71) were used to examine HP using immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR). Two different HP specific genes (23S rRNA and ureA) were used for TaqMan-based qPCR, and for subsequent verification using HP specific RIDAGENE HP kit and SYBR Green based qPCR. All of the OSCC specimens from both cohorts were found to be negative for HP infection with IHC and qPCR, although the positive control specimens tested positive. Our findings suggest that HP is absent in the examined OSCC cohorts, irrespective of race, lifestyle and habitual risk factors. This indicates that, in contrast to gastric cancer, HP is an unlikely contributing factor for OSCC pathogenesis. Nature Publishing Group UK 2020-05-26 /pmc/articles/PMC7250879/ /pubmed/32457404 http://dx.doi.org/10.1038/s41598-020-65694-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Pandey, Sushma
Follin-Arbelet, Benoit
Pun, Chin Bahadur
Gautam, Dej K.
Johannessen, Anne Christine
Petersen, Fernanda Cristina
Costea, Daniela Elena
Sapkota, Dipak
Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients
title Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients
title_full Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients
title_fullStr Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients
title_full_unstemmed Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients
title_short Helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of Norwegian and Nepalese patients
title_sort helicobacter pylori was not detected in oral squamous cell carcinomas from cohorts of norwegian and nepalese patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250879/
https://www.ncbi.nlm.nih.gov/pubmed/32457404
http://dx.doi.org/10.1038/s41598-020-65694-7
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