Cargando…
Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana
Chemical priming is an attractive and promising approach to improve abiotic stress tolerance in a broad variety of plant species. We screened the RIKEN Natural Products Depository (NPDepo) chemical library and identified a novel compound, FSL0260, enhancing salinity-stress tolerance in Arabidopsis t...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250896/ https://www.ncbi.nlm.nih.gov/pubmed/32457324 http://dx.doi.org/10.1038/s41598-020-65614-9 |
_version_ | 1783538848904511488 |
---|---|
author | Sako, Kaori Futamura, Yushi Shimizu, Takeshi Matsui, Akihiro Hirano, Hiroyuki Kondoh, Yasumitsu Muroi, Makoto Aono, Harumi Tanaka, Maho Honda, Kaori Shimizu, Kenshirou Kawatani, Makoto Nakano, Takeshi Osada, Hiroyuki Noguchi, Ko Seki, Motoaki |
author_facet | Sako, Kaori Futamura, Yushi Shimizu, Takeshi Matsui, Akihiro Hirano, Hiroyuki Kondoh, Yasumitsu Muroi, Makoto Aono, Harumi Tanaka, Maho Honda, Kaori Shimizu, Kenshirou Kawatani, Makoto Nakano, Takeshi Osada, Hiroyuki Noguchi, Ko Seki, Motoaki |
author_sort | Sako, Kaori |
collection | PubMed |
description | Chemical priming is an attractive and promising approach to improve abiotic stress tolerance in a broad variety of plant species. We screened the RIKEN Natural Products Depository (NPDepo) chemical library and identified a novel compound, FSL0260, enhancing salinity-stress tolerance in Arabidopsis thaliana and rice. Through transcriptome analysis using A. thaliana seedlings, treatment of FSL0260 elevated an alternative respiration pathway in mitochondria that modulates accumulation of reactive oxygen species (ROS). From comparison analysis, we realized that the alternative respiration pathway was induced by treatment of known mitochondrial inhibitors. We confirmed that known inhibitors of mitochondrial complex I, such as rotenone and piericidin A, also enhanced salt-stress tolerance in Arabidopsis. We demonstrated that FSL0260 binds to complex I of the mitochondrial electron transport chain and inhibits its activity, suggesting that inhibition of mitochondrial complex I activates an alternative respiration pathway resulting in reduction of ROS accumulation and enhancement of tolerance to salinity in plants. Furthermore, FSL0260 preferentially inhibited plant mitochondrial complex I rather than a mammalian complex, implying that FSL0260 has a potential to be an agent for improving salt-stress tolerance in agriculture that is low toxicity to humans. |
format | Online Article Text |
id | pubmed-7250896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72508962020-06-04 Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana Sako, Kaori Futamura, Yushi Shimizu, Takeshi Matsui, Akihiro Hirano, Hiroyuki Kondoh, Yasumitsu Muroi, Makoto Aono, Harumi Tanaka, Maho Honda, Kaori Shimizu, Kenshirou Kawatani, Makoto Nakano, Takeshi Osada, Hiroyuki Noguchi, Ko Seki, Motoaki Sci Rep Article Chemical priming is an attractive and promising approach to improve abiotic stress tolerance in a broad variety of plant species. We screened the RIKEN Natural Products Depository (NPDepo) chemical library and identified a novel compound, FSL0260, enhancing salinity-stress tolerance in Arabidopsis thaliana and rice. Through transcriptome analysis using A. thaliana seedlings, treatment of FSL0260 elevated an alternative respiration pathway in mitochondria that modulates accumulation of reactive oxygen species (ROS). From comparison analysis, we realized that the alternative respiration pathway was induced by treatment of known mitochondrial inhibitors. We confirmed that known inhibitors of mitochondrial complex I, such as rotenone and piericidin A, also enhanced salt-stress tolerance in Arabidopsis. We demonstrated that FSL0260 binds to complex I of the mitochondrial electron transport chain and inhibits its activity, suggesting that inhibition of mitochondrial complex I activates an alternative respiration pathway resulting in reduction of ROS accumulation and enhancement of tolerance to salinity in plants. Furthermore, FSL0260 preferentially inhibited plant mitochondrial complex I rather than a mammalian complex, implying that FSL0260 has a potential to be an agent for improving salt-stress tolerance in agriculture that is low toxicity to humans. Nature Publishing Group UK 2020-05-26 /pmc/articles/PMC7250896/ /pubmed/32457324 http://dx.doi.org/10.1038/s41598-020-65614-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Sako, Kaori Futamura, Yushi Shimizu, Takeshi Matsui, Akihiro Hirano, Hiroyuki Kondoh, Yasumitsu Muroi, Makoto Aono, Harumi Tanaka, Maho Honda, Kaori Shimizu, Kenshirou Kawatani, Makoto Nakano, Takeshi Osada, Hiroyuki Noguchi, Ko Seki, Motoaki Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana |
title | Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana |
title_full | Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana |
title_fullStr | Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana |
title_full_unstemmed | Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana |
title_short | Inhibition of mitochondrial complex I by the novel compound FSL0260 enhances high salinity-stress tolerance in Arabidopsis thaliana |
title_sort | inhibition of mitochondrial complex i by the novel compound fsl0260 enhances high salinity-stress tolerance in arabidopsis thaliana |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7250896/ https://www.ncbi.nlm.nih.gov/pubmed/32457324 http://dx.doi.org/10.1038/s41598-020-65614-9 |
work_keys_str_mv | AT sakokaori inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT futamurayushi inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT shimizutakeshi inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT matsuiakihiro inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT hiranohiroyuki inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT kondohyasumitsu inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT muroimakoto inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT aonoharumi inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT tanakamaho inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT hondakaori inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT shimizukenshirou inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT kawatanimakoto inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT nakanotakeshi inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT osadahiroyuki inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT noguchiko inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana AT sekimotoaki inhibitionofmitochondrialcomplexibythenovelcompoundfsl0260enhanceshighsalinitystresstoleranceinarabidopsisthaliana |