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G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan
Rotavirus A (RVA) is a diarrheal pathogen affecting children under age five, particularly in developing and underdeveloped regions of the world due to malnutrition, poor healthcare and hygienic conditions. Water and food contamination are found to be major sources of diarrheal outbreaks. Pakistan is...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251132/ https://www.ncbi.nlm.nih.gov/pubmed/32457481 http://dx.doi.org/10.1038/s41598-020-65583-z |
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author | Naqvi, Syeda Sumera Javed, Sundus Naseem, Saadia Sadiq, Asma Khan, Netasha Sattar, Sadia Shah, Naseer Ali Bostan, Nazish |
author_facet | Naqvi, Syeda Sumera Javed, Sundus Naseem, Saadia Sadiq, Asma Khan, Netasha Sattar, Sadia Shah, Naseer Ali Bostan, Nazish |
author_sort | Naqvi, Syeda Sumera |
collection | PubMed |
description | Rotavirus A (RVA) is a diarrheal pathogen affecting children under age five, particularly in developing and underdeveloped regions of the world due to malnutrition, poor healthcare and hygienic conditions. Water and food contamination are found to be major sources of diarrheal outbreaks. Pakistan is one of the countries with high RVA related diarrhea burden but with insufficient surveillance system. The aim of this study was to gauge the RVA contamination of major open sewerage collecting streams and household water supplies in two major metropolitan cities of Pakistan. Three concentration methods were compared using RNA purity and concentration as parameters, and detection efficiency of the selected method was estimated. Water samples were collected from 21 sites in Islamabad and Rawalpindi in two phases during the year 2014–2015. Meteorological conditions were recorded for each sampling day and site from Pakistan Meteorological Department (PMD). Nested PCR was used to detect the presence of RVA in samples targeting the VP7 gene. Logistic regression was applied to assess the association of weather conditions with RVA persistence in water bodies. Statistical analysis hinted at a temporal and seasonal pattern of RVA detection in water. Phylogenetic analysis of selected isolates showed a close association of environmental strains with clinical RVA isolates from hospitalized children with acute diarrhea during the same period. This is the first scientific report cataloging the circulating RVA strains in environmental samples from the region. The study highlights the hazards of releasing untreated sewerage containing potentially infectious viral particles into collecting streams, which could become a reservoir of multiple pathogens and a risk to exposed communities. Moreover, routine testing of these water bodies can present an effective surveillance system of circulating viral strains in the population. |
format | Online Article Text |
id | pubmed-7251132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72511322020-06-04 G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan Naqvi, Syeda Sumera Javed, Sundus Naseem, Saadia Sadiq, Asma Khan, Netasha Sattar, Sadia Shah, Naseer Ali Bostan, Nazish Sci Rep Article Rotavirus A (RVA) is a diarrheal pathogen affecting children under age five, particularly in developing and underdeveloped regions of the world due to malnutrition, poor healthcare and hygienic conditions. Water and food contamination are found to be major sources of diarrheal outbreaks. Pakistan is one of the countries with high RVA related diarrhea burden but with insufficient surveillance system. The aim of this study was to gauge the RVA contamination of major open sewerage collecting streams and household water supplies in two major metropolitan cities of Pakistan. Three concentration methods were compared using RNA purity and concentration as parameters, and detection efficiency of the selected method was estimated. Water samples were collected from 21 sites in Islamabad and Rawalpindi in two phases during the year 2014–2015. Meteorological conditions were recorded for each sampling day and site from Pakistan Meteorological Department (PMD). Nested PCR was used to detect the presence of RVA in samples targeting the VP7 gene. Logistic regression was applied to assess the association of weather conditions with RVA persistence in water bodies. Statistical analysis hinted at a temporal and seasonal pattern of RVA detection in water. Phylogenetic analysis of selected isolates showed a close association of environmental strains with clinical RVA isolates from hospitalized children with acute diarrhea during the same period. This is the first scientific report cataloging the circulating RVA strains in environmental samples from the region. The study highlights the hazards of releasing untreated sewerage containing potentially infectious viral particles into collecting streams, which could become a reservoir of multiple pathogens and a risk to exposed communities. Moreover, routine testing of these water bodies can present an effective surveillance system of circulating viral strains in the population. Nature Publishing Group UK 2020-05-26 /pmc/articles/PMC7251132/ /pubmed/32457481 http://dx.doi.org/10.1038/s41598-020-65583-z Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Naqvi, Syeda Sumera Javed, Sundus Naseem, Saadia Sadiq, Asma Khan, Netasha Sattar, Sadia Shah, Naseer Ali Bostan, Nazish G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan |
title | G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan |
title_full | G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan |
title_fullStr | G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan |
title_full_unstemmed | G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan |
title_short | G3 and G9 Rotavirus genotypes in waste water circulation from two major metropolitan cities of Pakistan |
title_sort | g3 and g9 rotavirus genotypes in waste water circulation from two major metropolitan cities of pakistan |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251132/ https://www.ncbi.nlm.nih.gov/pubmed/32457481 http://dx.doi.org/10.1038/s41598-020-65583-z |
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