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Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System

Genome editing of human cluster of differentiation 34(+) (CD34(+)) hematopoietic stem and progenitor cells (HSPCs) holds great therapeutic potential. This study aimed to optimize on-target, ex vivo genome editing using the CRISPR-Cas9 system in CD34(+) HSPCs and to create a clear workflow for precis...

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Detalles Bibliográficos
Autores principales:	Shapiro, Jenny, Iancu, Ortal, Jacobi, Ashley M., McNeill, Matthew S., Turk, Rolf, Rettig, Garrett R., Amit, Ido, Tovin-Recht, Adi, Yakhini, Zohar, Behlke, Mark A., Hendel, Ayal
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251314/ https://www.ncbi.nlm.nih.gov/pubmed/32478125 http://dx.doi.org/10.1016/j.omtm.2020.04.027

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