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Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis

Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to thre...

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Autores principales: Hwang, Jung Eun, Hwang, Sun-Goo, Jung, In Jung, Han, Sung Min, Ahn, Joon-Woo, Kim, Jin-Baek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251472/
https://www.ncbi.nlm.nih.gov/pubmed/31479093
http://dx.doi.org/10.1590/1678-4685-GMB-2018-0273
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author Hwang, Jung Eun
Hwang, Sun-Goo
Jung, In Jung
Han, Sung Min
Ahn, Joon-Woo
Kim, Jin-Baek
author_facet Hwang, Jung Eun
Hwang, Sun-Goo
Jung, In Jung
Han, Sung Min
Ahn, Joon-Woo
Kim, Jin-Baek
author_sort Hwang, Jung Eun
collection PubMed
description Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322 expression on radiation response, OsFBX322 was overexpressed in Arabidopsis. Transgenic overexpression lines were more sensitive to gamma irradiation than control plants. These results suggest that OsFBX322 plays a negative role in the defense response to radiation in plants. In addition, we obtained four co-expression genes of OsFBX322 by specific co-expression networks using the ARANCE. Quantitative RT-PCR showed that the four genes were also downregulated after exposure to the three types of radiation. These results imply that the co-expressed genes may serve as key regulators in the radiation response pathway in plants.
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spelling pubmed-72514722020-06-08 Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis Hwang, Jung Eun Hwang, Sun-Goo Jung, In Jung Han, Sung Min Ahn, Joon-Woo Kim, Jin-Baek Genet Mol Biol Plant Genetics Ionizing radiation has a substantial effect on physiological and biochemical processes in plants via induction of transcriptional changes and diverse genetic alterations. Previous microarray analysis showed that rice OsFBX322, which encodes a rice F-box protein, was downregulated in response to three types of ionizing radiation: gamma irradiation, ion beams, and cosmic rays. In order to characterize the radiation-responsive genes in rice, OsFBX322 was selected for further analysis. OsFBX322 expression patterns in response to radiation were confirmed using quantitative RT-PCR. Transient expression of a GFP-OsFBX322 fusion protein in tobacco leaf epidermis indicated that OsFBX322 is localized to the nucleus. To determine the effect of OsFBX322 expression on radiation response, OsFBX322 was overexpressed in Arabidopsis. Transgenic overexpression lines were more sensitive to gamma irradiation than control plants. These results suggest that OsFBX322 plays a negative role in the defense response to radiation in plants. In addition, we obtained four co-expression genes of OsFBX322 by specific co-expression networks using the ARANCE. Quantitative RT-PCR showed that the four genes were also downregulated after exposure to the three types of radiation. These results imply that the co-expressed genes may serve as key regulators in the radiation response pathway in plants. Sociedade Brasileira de Genética 2020-03-02 /pmc/articles/PMC7251472/ /pubmed/31479093 http://dx.doi.org/10.1590/1678-4685-GMB-2018-0273 Text en Copyright © 2020, Sociedade Brasileira de Genética. https://creativecommons.org/licenses/by/4.0/ License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License (type CC-BY), which permits unrestricted use, distribution and reproduction in any medium, provided the original article is properly cited.
spellingShingle Plant Genetics
Hwang, Jung Eun
Hwang, Sun-Goo
Jung, In Jung
Han, Sung Min
Ahn, Joon-Woo
Kim, Jin-Baek
Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
title Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
title_full Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
title_fullStr Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
title_full_unstemmed Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
title_short Overexpression of rice F-box protein OsFBX322 confers increased sensitivity to gamma irradiation in Arabidopsis
title_sort overexpression of rice f-box protein osfbx322 confers increased sensitivity to gamma irradiation in arabidopsis
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251472/
https://www.ncbi.nlm.nih.gov/pubmed/31479093
http://dx.doi.org/10.1590/1678-4685-GMB-2018-0273
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