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Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition

Matrix metalloproteinases (MMPs) are involved in the cleavage of several components of the extracellular matrix and serve important roles in tumor growth, metastasis and invasion. Previous studies have focused on the expression of one or several MMPs in esophageal squamous cell carcinoma (ESCC); how...

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Autores principales: Xu, Guifeng, Ou, Ling, Liu, Ying, Wang, Xiao, Liu, Kaisheng, Li, Jieling, Li, Junjun, Wang, Shaoqi, Huang, Dane, Zheng, Kai, Wang, Shaoxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251684/
https://www.ncbi.nlm.nih.gov/pubmed/32627007
http://dx.doi.org/10.3892/or.2020.7606
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author Xu, Guifeng
Ou, Ling
Liu, Ying
Wang, Xiao
Liu, Kaisheng
Li, Jieling
Li, Junjun
Wang, Shaoqi
Huang, Dane
Zheng, Kai
Wang, Shaoxiang
author_facet Xu, Guifeng
Ou, Ling
Liu, Ying
Wang, Xiao
Liu, Kaisheng
Li, Jieling
Li, Junjun
Wang, Shaoqi
Huang, Dane
Zheng, Kai
Wang, Shaoxiang
author_sort Xu, Guifeng
collection PubMed
description Matrix metalloproteinases (MMPs) are involved in the cleavage of several components of the extracellular matrix and serve important roles in tumor growth, metastasis and invasion. Previous studies have focused on the expression of one or several MMPs in esophageal squamous cell carcinoma (ESCC); however, in the present study, the transcriptomics of all 23 MMPs were systematically investigated with a focus on the prognostic value of the combination of MMPs. In this study, 8 overlapping differentially expressed genes of the MMP family were identified based on data obtained from Gene Expression Omnibus and The Cancer Genome Atlas. The prognostic value of these MMPs were investigated; the receiver operating characteristic curves, survival curves and nomograms showed that the combination of 6 selected MMPs possessed a good predictive ability, which was more accurate than the prediction model based on Tumor-Node-Metastasis stage. Gene set enrichment analysis and gene co-expression analysis were performed to investigate the potential mechanism of action of MMPs in ESCC. The MMP family was associated with several signaling pathways, such as epithelial-mesenchymal transition (EMT), Notch, TGF-β, mTOR and P53. Cell Counting Kit-8, colony formation, wound healing assays and western blotting were used to determine the effect of BB-94, a pan-MMP inhibitor, on proliferation and migration of ESCC cells. BB-94 treatment decreased ESCC cell growth, migration and EMT. Therefore, MMPs may serve both as diagnostic and prognostic biomarkers of ESCC, and MMP inhibition may be a promising preventive and therapeutic strategy for patients with ESCC.
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spelling pubmed-72516842020-05-28 Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition Xu, Guifeng Ou, Ling Liu, Ying Wang, Xiao Liu, Kaisheng Li, Jieling Li, Junjun Wang, Shaoqi Huang, Dane Zheng, Kai Wang, Shaoxiang Oncol Rep Articles Matrix metalloproteinases (MMPs) are involved in the cleavage of several components of the extracellular matrix and serve important roles in tumor growth, metastasis and invasion. Previous studies have focused on the expression of one or several MMPs in esophageal squamous cell carcinoma (ESCC); however, in the present study, the transcriptomics of all 23 MMPs were systematically investigated with a focus on the prognostic value of the combination of MMPs. In this study, 8 overlapping differentially expressed genes of the MMP family were identified based on data obtained from Gene Expression Omnibus and The Cancer Genome Atlas. The prognostic value of these MMPs were investigated; the receiver operating characteristic curves, survival curves and nomograms showed that the combination of 6 selected MMPs possessed a good predictive ability, which was more accurate than the prediction model based on Tumor-Node-Metastasis stage. Gene set enrichment analysis and gene co-expression analysis were performed to investigate the potential mechanism of action of MMPs in ESCC. The MMP family was associated with several signaling pathways, such as epithelial-mesenchymal transition (EMT), Notch, TGF-β, mTOR and P53. Cell Counting Kit-8, colony formation, wound healing assays and western blotting were used to determine the effect of BB-94, a pan-MMP inhibitor, on proliferation and migration of ESCC cells. BB-94 treatment decreased ESCC cell growth, migration and EMT. Therefore, MMPs may serve both as diagnostic and prognostic biomarkers of ESCC, and MMP inhibition may be a promising preventive and therapeutic strategy for patients with ESCC. D.A. Spandidos 2020-07 2020-05-07 /pmc/articles/PMC7251684/ /pubmed/32627007 http://dx.doi.org/10.3892/or.2020.7606 Text en Copyright: © Xu et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Xu, Guifeng
Ou, Ling
Liu, Ying
Wang, Xiao
Liu, Kaisheng
Li, Jieling
Li, Junjun
Wang, Shaoqi
Huang, Dane
Zheng, Kai
Wang, Shaoxiang
Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
title Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
title_full Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
title_fullStr Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
title_full_unstemmed Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
title_short Upregulated expression of MMP family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
title_sort upregulated expression of mmp family genes is associated with poor survival in patients with esophageal squamous cell carcinoma via regulation of proliferation and epithelial-mesenchymal transition
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251684/
https://www.ncbi.nlm.nih.gov/pubmed/32627007
http://dx.doi.org/10.3892/or.2020.7606
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