FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing

Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution. By quantifying BrdU incorporation along pulse-chased replication intermed...

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Detalles Bibliográficos
Autores principales: Hennion, Magali, Arbona, Jean-Michel, Lacroix, Laurent, Cruaud, Corinne, Theulot, Bertrand, Tallec, Benoît Le, Proux, Florence, Wu, Xia, Novikova, Elizaveta, Engelen, Stefan, Lemainque, Arnaud, Audit, Benjamin, Hyrien, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251829/
https://www.ncbi.nlm.nih.gov/pubmed/32456659
http://dx.doi.org/10.1186/s13059-020-02013-3

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