A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum

The CRISPR/Cas9 system enables precise genome editing and is a useful tool for functional genomic studies. Here we report a detailed protocol for targeted genome editing in the model grass Brachypodium distachyon and its allotetraploid relative B. hybridum, describing gRNA design, a transient protop...

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Detalles Bibliográficos
Autores principales: Hus, Karolina, Betekhtin, Alexander, Pinski, Artur, Rojek-Jelonek, Magdalena, Grzebelus, Ewa, Nibau, Candida, Gao, Mingjun, Jaeger, Katja E., Jenkins, Glyn, Doonan, John H., Hasterok, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7251944/
https://www.ncbi.nlm.nih.gov/pubmed/32508865
http://dx.doi.org/10.3389/fpls.2020.00614

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