A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue

Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume...

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Autores principales: Wei, Shan, Levy, Brynn, Hoffman, Nataly, Cujar, Claudia, Rodney-Sandy, Reunet, Wapner, Ronald, D'Alton, Mary, Williams, Zev
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252492/
https://www.ncbi.nlm.nih.gov/pubmed/32054310
http://dx.doi.org/10.2144/btn-2019-0172
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author Wei, Shan
Levy, Brynn
Hoffman, Nataly
Cujar, Claudia
Rodney-Sandy, Reunet
Wapner, Ronald
D'Alton, Mary
Williams, Zev
author_facet Wei, Shan
Levy, Brynn
Hoffman, Nataly
Cujar, Claudia
Rodney-Sandy, Reunet
Wapner, Ronald
D'Alton, Mary
Williams, Zev
author_sort Wei, Shan
collection PubMed
description Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.
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spelling pubmed-72524922020-05-28 A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue Wei, Shan Levy, Brynn Hoffman, Nataly Cujar, Claudia Rodney-Sandy, Reunet Wapner, Ronald D'Alton, Mary Williams, Zev Biotechniques Reports Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications. Future Science Ltd 2020-02-14 2020-05 /pmc/articles/PMC7252492/ /pubmed/32054310 http://dx.doi.org/10.2144/btn-2019-0172 Text en © 2020 Zev Williams This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Reports
Wei, Shan
Levy, Brynn
Hoffman, Nataly
Cujar, Claudia
Rodney-Sandy, Reunet
Wapner, Ronald
D'Alton, Mary
Williams, Zev
A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
title A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
title_full A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
title_fullStr A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
title_full_unstemmed A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
title_short A rapid and simple bead-bashing-based method for genomic DNA extraction from mammalian tissue
title_sort rapid and simple bead-bashing-based method for genomic dna extraction from mammalian tissue
topic Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252492/
https://www.ncbi.nlm.nih.gov/pubmed/32054310
http://dx.doi.org/10.2144/btn-2019-0172
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