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Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea

The dynamics of diatoms and dinoflagellates have been monitored for many decades at the Helgoland Roads Long-Term Ecological Research site and are relatively well understood. In contrast, small-sized eukaryotic microbes and their community changes are still much more elusive, mainly due to their sma...

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Autores principales: Käse, Laura, Kraberg, Alexandra C, Metfies, Katja, Neuhaus, Stefan, Sprong, Pim A A, Fuchs, Bernhard M, Boersma, Maarten, Wiltshire, Karen H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252501/
https://www.ncbi.nlm.nih.gov/pubmed/32494090
http://dx.doi.org/10.1093/plankt/fbaa017
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author Käse, Laura
Kraberg, Alexandra C
Metfies, Katja
Neuhaus, Stefan
Sprong, Pim A A
Fuchs, Bernhard M
Boersma, Maarten
Wiltshire, Karen H
author_facet Käse, Laura
Kraberg, Alexandra C
Metfies, Katja
Neuhaus, Stefan
Sprong, Pim A A
Fuchs, Bernhard M
Boersma, Maarten
Wiltshire, Karen H
author_sort Käse, Laura
collection PubMed
description The dynamics of diatoms and dinoflagellates have been monitored for many decades at the Helgoland Roads Long-Term Ecological Research site and are relatively well understood. In contrast, small-sized eukaryotic microbes and their community changes are still much more elusive, mainly due to their small size and uniform morphology, which makes them difficult to identify microscopically. By using next-generation sequencing, we wanted to shed light on the Helgoland planktonic community dynamics, including nano- and picoplankton, during a spring bloom. We took samples from March to May 2016 and sequenced the V4 region of the 18S rDNA. Our results showed that mixotrophic and heterotrophic taxa were more abundant than autotrophic diatoms. Dinoflagellates dominated the sequence assemblage, and several small-sized eukaryotic microbes like Haptophyta, Choanoflagellata, Marine Stramenopiles and Syndiniales were identified. A diverse background community including taxa from all size classes was present during the whole sampling period. Five phases with several communities were distinguished. The fastest changes in community composition took place in phase 3, while the communities from phases 1 to 5 were more similar to each other despite contrasting environmental conditions. Synergy effects of next-generation sequencing and traditional methods may be exploited in future long-term observations.
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spelling pubmed-72525012020-06-02 Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea Käse, Laura Kraberg, Alexandra C Metfies, Katja Neuhaus, Stefan Sprong, Pim A A Fuchs, Bernhard M Boersma, Maarten Wiltshire, Karen H J Plankton Res Original Article The dynamics of diatoms and dinoflagellates have been monitored for many decades at the Helgoland Roads Long-Term Ecological Research site and are relatively well understood. In contrast, small-sized eukaryotic microbes and their community changes are still much more elusive, mainly due to their small size and uniform morphology, which makes them difficult to identify microscopically. By using next-generation sequencing, we wanted to shed light on the Helgoland planktonic community dynamics, including nano- and picoplankton, during a spring bloom. We took samples from March to May 2016 and sequenced the V4 region of the 18S rDNA. Our results showed that mixotrophic and heterotrophic taxa were more abundant than autotrophic diatoms. Dinoflagellates dominated the sequence assemblage, and several small-sized eukaryotic microbes like Haptophyta, Choanoflagellata, Marine Stramenopiles and Syndiniales were identified. A diverse background community including taxa from all size classes was present during the whole sampling period. Five phases with several communities were distinguished. The fastest changes in community composition took place in phase 3, while the communities from phases 1 to 5 were more similar to each other despite contrasting environmental conditions. Synergy effects of next-generation sequencing and traditional methods may be exploited in future long-term observations. Oxford University Press 2020-05-14 /pmc/articles/PMC7252501/ /pubmed/32494090 http://dx.doi.org/10.1093/plankt/fbaa017 Text en © The Author(s) 2020. Published by Oxford University Press. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Käse, Laura
Kraberg, Alexandra C
Metfies, Katja
Neuhaus, Stefan
Sprong, Pim A A
Fuchs, Bernhard M
Boersma, Maarten
Wiltshire, Karen H
Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea
title Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea
title_full Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea
title_fullStr Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea
title_full_unstemmed Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea
title_short Rapid succession drives spring community dynamics of small protists at Helgoland Roads, North Sea
title_sort rapid succession drives spring community dynamics of small protists at helgoland roads, north sea
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7252501/
https://www.ncbi.nlm.nih.gov/pubmed/32494090
http://dx.doi.org/10.1093/plankt/fbaa017
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