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OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae

BACKGROUND: Bacterial leaf streak (BLS) and bacterial blight (BB) are two major prevalent and devastating rice bacterial diseases caused by the Gram-negative bacteria of Xanthomonas oryzae pv. oryzicola (Xoc) and Xanthomonas oryzae pv. oryzae (Xoo), respectively. Previously, we identified a defence-...

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Detalles Bibliográficos
Autores principales: Yang, Wei, Ju, Yanhu, Zuo, Liping, Shang, Luyue, Li, Xinru, Li, Xiaoming, Feng, Shangzong, Ding, Xinhua, Chu, Zhaohui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253548/
https://www.ncbi.nlm.nih.gov/pubmed/32462553
http://dx.doi.org/10.1186/s12284-020-00388-2
Descripción
Sumario:BACKGROUND: Bacterial leaf streak (BLS) and bacterial blight (BB) are two major prevalent and devastating rice bacterial diseases caused by the Gram-negative bacteria of Xanthomonas oryzae pv. oryzicola (Xoc) and Xanthomonas oryzae pv. oryzae (Xoo), respectively. Previously, we identified a defence-related (DR) gene encoding a small heat shock protein, OsHsp18.0-CI, that positively regulates BLS and BB resistance in rice. RESULTS: To reveal the regulatory mechanism of the OsHsp18.0-CI response to Xoc and Xoo, we characterized the class B heat shock factor (Hsf), OsHsfB4d, through transcriptional analysis and a transgenic study. OsHsfB4d is upregulated post inoculation by either the Xoc strain RS105 or Xoo strain PXO99a in Zhonghua 11 (wild type, ZH11) as well as in OsHsp18.0-CI overexpressing rice plants. Transient expression of OsHsfB4d can activate the expression of green fluorescent protein (GFP) and luciferase (Luc) via the OsHsp18.0-CI promoter. Rice plants overexpressing OsHsfB4d exhibited enhanced resistance to RS105 and PXO99a as well as increased expression of OsHsp18.0-CI and pathogenesis-related genes. Furthermore, we found that OsHsfB4d directly binds to a DNA fragment carrying the only perfect heat shock element (HSE) in the promoter of OsHsp18.0-CI. CONCLUSION: Overall, we reveal that OsHsfB4d, a class B Hsf, acts as a positive regulator of OsHsp18.0-CI to mediate BLS and BB resistance in rice.