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OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae
BACKGROUND: Bacterial leaf streak (BLS) and bacterial blight (BB) are two major prevalent and devastating rice bacterial diseases caused by the Gram-negative bacteria of Xanthomonas oryzae pv. oryzicola (Xoc) and Xanthomonas oryzae pv. oryzae (Xoo), respectively. Previously, we identified a defence-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253548/ https://www.ncbi.nlm.nih.gov/pubmed/32462553 http://dx.doi.org/10.1186/s12284-020-00388-2 |
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author | Yang, Wei Ju, Yanhu Zuo, Liping Shang, Luyue Li, Xinru Li, Xiaoming Feng, Shangzong Ding, Xinhua Chu, Zhaohui |
author_facet | Yang, Wei Ju, Yanhu Zuo, Liping Shang, Luyue Li, Xinru Li, Xiaoming Feng, Shangzong Ding, Xinhua Chu, Zhaohui |
author_sort | Yang, Wei |
collection | PubMed |
description | BACKGROUND: Bacterial leaf streak (BLS) and bacterial blight (BB) are two major prevalent and devastating rice bacterial diseases caused by the Gram-negative bacteria of Xanthomonas oryzae pv. oryzicola (Xoc) and Xanthomonas oryzae pv. oryzae (Xoo), respectively. Previously, we identified a defence-related (DR) gene encoding a small heat shock protein, OsHsp18.0-CI, that positively regulates BLS and BB resistance in rice. RESULTS: To reveal the regulatory mechanism of the OsHsp18.0-CI response to Xoc and Xoo, we characterized the class B heat shock factor (Hsf), OsHsfB4d, through transcriptional analysis and a transgenic study. OsHsfB4d is upregulated post inoculation by either the Xoc strain RS105 or Xoo strain PXO99a in Zhonghua 11 (wild type, ZH11) as well as in OsHsp18.0-CI overexpressing rice plants. Transient expression of OsHsfB4d can activate the expression of green fluorescent protein (GFP) and luciferase (Luc) via the OsHsp18.0-CI promoter. Rice plants overexpressing OsHsfB4d exhibited enhanced resistance to RS105 and PXO99a as well as increased expression of OsHsp18.0-CI and pathogenesis-related genes. Furthermore, we found that OsHsfB4d directly binds to a DNA fragment carrying the only perfect heat shock element (HSE) in the promoter of OsHsp18.0-CI. CONCLUSION: Overall, we reveal that OsHsfB4d, a class B Hsf, acts as a positive regulator of OsHsp18.0-CI to mediate BLS and BB resistance in rice. |
format | Online Article Text |
id | pubmed-7253548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-72535482020-06-08 OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae Yang, Wei Ju, Yanhu Zuo, Liping Shang, Luyue Li, Xinru Li, Xiaoming Feng, Shangzong Ding, Xinhua Chu, Zhaohui Rice (N Y) Original Article BACKGROUND: Bacterial leaf streak (BLS) and bacterial blight (BB) are two major prevalent and devastating rice bacterial diseases caused by the Gram-negative bacteria of Xanthomonas oryzae pv. oryzicola (Xoc) and Xanthomonas oryzae pv. oryzae (Xoo), respectively. Previously, we identified a defence-related (DR) gene encoding a small heat shock protein, OsHsp18.0-CI, that positively regulates BLS and BB resistance in rice. RESULTS: To reveal the regulatory mechanism of the OsHsp18.0-CI response to Xoc and Xoo, we characterized the class B heat shock factor (Hsf), OsHsfB4d, through transcriptional analysis and a transgenic study. OsHsfB4d is upregulated post inoculation by either the Xoc strain RS105 or Xoo strain PXO99a in Zhonghua 11 (wild type, ZH11) as well as in OsHsp18.0-CI overexpressing rice plants. Transient expression of OsHsfB4d can activate the expression of green fluorescent protein (GFP) and luciferase (Luc) via the OsHsp18.0-CI promoter. Rice plants overexpressing OsHsfB4d exhibited enhanced resistance to RS105 and PXO99a as well as increased expression of OsHsp18.0-CI and pathogenesis-related genes. Furthermore, we found that OsHsfB4d directly binds to a DNA fragment carrying the only perfect heat shock element (HSE) in the promoter of OsHsp18.0-CI. CONCLUSION: Overall, we reveal that OsHsfB4d, a class B Hsf, acts as a positive regulator of OsHsp18.0-CI to mediate BLS and BB resistance in rice. Springer US 2020-05-27 /pmc/articles/PMC7253548/ /pubmed/32462553 http://dx.doi.org/10.1186/s12284-020-00388-2 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Original Article Yang, Wei Ju, Yanhu Zuo, Liping Shang, Luyue Li, Xinru Li, Xiaoming Feng, Shangzong Ding, Xinhua Chu, Zhaohui OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae |
title | OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae |
title_full | OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae |
title_fullStr | OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae |
title_full_unstemmed | OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae |
title_short | OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae |
title_sort | oshsfb4d binds the promoter and regulates the expression of oshsp18.0-ci to resistant against xanthomonas oryzae |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253548/ https://www.ncbi.nlm.nih.gov/pubmed/32462553 http://dx.doi.org/10.1186/s12284-020-00388-2 |
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