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Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand
Among the complex microbial community living in the mosquito midgut, some bacteria (e.g., Enterobacter spp.) can deliver effector molecules with anti-Plasmodium effects suppressing the development of malaria parasites (Plasmodium falciparum) before the öokinete can penetrate the mosquito midgut epit...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253650/ https://www.ncbi.nlm.nih.gov/pubmed/32508784 http://dx.doi.org/10.3389/fmicb.2020.00965 |
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author | Tainchum, Krajana Dupont, Chloé Chareonviriyaphap, Theeraphap Jumas-Bilak, Estelle Bangs, Michael J. Manguin, Sylvie |
author_facet | Tainchum, Krajana Dupont, Chloé Chareonviriyaphap, Theeraphap Jumas-Bilak, Estelle Bangs, Michael J. Manguin, Sylvie |
author_sort | Tainchum, Krajana |
collection | PubMed |
description | Among the complex microbial community living in the mosquito midgut, some bacteria (e.g., Enterobacter spp.) can deliver effector molecules with anti-Plasmodium effects suppressing the development of malaria parasites (Plasmodium falciparum) before the öokinete can penetrate the mosquito midgut epithelium. Despite knowledge of this phenomenon, only a few studies have defined the diversity of microbiota in wild-caught adult Anopheles species. The objective of this study was to analyze and compare the bacterial microbiota in different Anopheles species, including representatives of the primary malaria vectors in western Thailand. Wild female Anopheles species were sampled from malaria-endemic areas in Tak and Mae Hong Son provinces near the Thai-Myanmar border. Midgut/abdominal bacterial diversity was assessed by examining the 16S rRNA gene, V3 hypervariable region, using PCR-Temporal Temperature Gel Electrophoresis (PCR-TTGE) profiling and sequence analysis. A total of 24 bacterial genera were identified from eight Anopheles species. Five bacterial genera were newly reported in Anopheles mosquitoes (Ferrimonas, Megasphaera, Pectobacterium, Shimwellia, and Trabulsiella). Five genera, including Megasphaera, were detected exclusively in a single-malaria (Plasmodium vivax) infected Anopheles minimus and not observed in other non-infected mosquitoes. The use of PCR-TTGE provides the first characterization of the midgut bacterial microbiome present in wild adult Anopheles in Thailand. Evidence that microbiota might impact pathogen development (suppression) in Anopheles and thereby reduce the risk of pathogen transmission deserves more studies to describe the presence and better understand the biological role of bacteria in natural mosquito populations. |
format | Online Article Text |
id | pubmed-7253650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72536502020-06-05 Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand Tainchum, Krajana Dupont, Chloé Chareonviriyaphap, Theeraphap Jumas-Bilak, Estelle Bangs, Michael J. Manguin, Sylvie Front Microbiol Microbiology Among the complex microbial community living in the mosquito midgut, some bacteria (e.g., Enterobacter spp.) can deliver effector molecules with anti-Plasmodium effects suppressing the development of malaria parasites (Plasmodium falciparum) before the öokinete can penetrate the mosquito midgut epithelium. Despite knowledge of this phenomenon, only a few studies have defined the diversity of microbiota in wild-caught adult Anopheles species. The objective of this study was to analyze and compare the bacterial microbiota in different Anopheles species, including representatives of the primary malaria vectors in western Thailand. Wild female Anopheles species were sampled from malaria-endemic areas in Tak and Mae Hong Son provinces near the Thai-Myanmar border. Midgut/abdominal bacterial diversity was assessed by examining the 16S rRNA gene, V3 hypervariable region, using PCR-Temporal Temperature Gel Electrophoresis (PCR-TTGE) profiling and sequence analysis. A total of 24 bacterial genera were identified from eight Anopheles species. Five bacterial genera were newly reported in Anopheles mosquitoes (Ferrimonas, Megasphaera, Pectobacterium, Shimwellia, and Trabulsiella). Five genera, including Megasphaera, were detected exclusively in a single-malaria (Plasmodium vivax) infected Anopheles minimus and not observed in other non-infected mosquitoes. The use of PCR-TTGE provides the first characterization of the midgut bacterial microbiome present in wild adult Anopheles in Thailand. Evidence that microbiota might impact pathogen development (suppression) in Anopheles and thereby reduce the risk of pathogen transmission deserves more studies to describe the presence and better understand the biological role of bacteria in natural mosquito populations. Frontiers Media S.A. 2020-05-21 /pmc/articles/PMC7253650/ /pubmed/32508784 http://dx.doi.org/10.3389/fmicb.2020.00965 Text en Copyright © 2020 Tainchum, Dupont, Chareonviriyaphap, Jumas-Bilak, Bangs and Manguin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Tainchum, Krajana Dupont, Chloé Chareonviriyaphap, Theeraphap Jumas-Bilak, Estelle Bangs, Michael J. Manguin, Sylvie Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand |
title | Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand |
title_full | Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand |
title_fullStr | Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand |
title_full_unstemmed | Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand |
title_short | Bacterial Microbiome in Wild-Caught Anopheles Mosquitoes in Western Thailand |
title_sort | bacterial microbiome in wild-caught anopheles mosquitoes in western thailand |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253650/ https://www.ncbi.nlm.nih.gov/pubmed/32508784 http://dx.doi.org/10.3389/fmicb.2020.00965 |
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