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A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation

BACKGROUND: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time o...

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Autores principales: Zarrin, Yasaman, Bakhteyari, Abbas, Nikpour, Parvaneh, Mostafavi, Fatemeh Sadat, Eskandari, Nahid, Matinfar, Mohammad, Aboutorabi, Roshanak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253937/
https://www.ncbi.nlm.nih.gov/pubmed/32500011
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author Zarrin, Yasaman
Bakhteyari, Abbas
Nikpour, Parvaneh
Mostafavi, Fatemeh Sadat
Eskandari, Nahid
Matinfar, Mohammad
Aboutorabi, Roshanak
author_facet Zarrin, Yasaman
Bakhteyari, Abbas
Nikpour, Parvaneh
Mostafavi, Fatemeh Sadat
Eskandari, Nahid
Matinfar, Mohammad
Aboutorabi, Roshanak
author_sort Zarrin, Yasaman
collection PubMed
description BACKGROUND: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time of endometrial receptivity. METHODS: Twenty-eight female rats were divided into control, diabetic, pioglitazone-treated and metformin-treated groups. Western blot was performed to determine the OPN and α3β1 integrin proteins in rats’ endometrium at the time of implantation. Data were analyzed by analysis of variance (ANOVA) and p<0.05 was considered statistically significant. RESULTS: OPN increased significantly in the diabetic group in comparison with control (p<0.001), metformin-treated (p=0.008) and pioglitazone-treated groups (p< 0.001). Furthermore, α3β1 integrin protein level in diabetic group had a significant difference in comparison with that of the control (p<0.001), metformin-treated (p= 0.026) and pioglitazone-treated groups (p<0.001). CONCLUSION: OPN and α3β1 integrin proteins are involved in embryo implantation and their changes in diabetic condition can affect fertility. Treatment with pioglitazone and metformin improved the level of OPN and α3β1 integrin proteins while pioglitazone was more effective.
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spelling pubmed-72539372020-06-03 A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation Zarrin, Yasaman Bakhteyari, Abbas Nikpour, Parvaneh Mostafavi, Fatemeh Sadat Eskandari, Nahid Matinfar, Mohammad Aboutorabi, Roshanak J Reprod Infertil Original Article BACKGROUND: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time of endometrial receptivity. METHODS: Twenty-eight female rats were divided into control, diabetic, pioglitazone-treated and metformin-treated groups. Western blot was performed to determine the OPN and α3β1 integrin proteins in rats’ endometrium at the time of implantation. Data were analyzed by analysis of variance (ANOVA) and p<0.05 was considered statistically significant. RESULTS: OPN increased significantly in the diabetic group in comparison with control (p<0.001), metformin-treated (p=0.008) and pioglitazone-treated groups (p< 0.001). Furthermore, α3β1 integrin protein level in diabetic group had a significant difference in comparison with that of the control (p<0.001), metformin-treated (p= 0.026) and pioglitazone-treated groups (p<0.001). CONCLUSION: OPN and α3β1 integrin proteins are involved in embryo implantation and their changes in diabetic condition can affect fertility. Treatment with pioglitazone and metformin improved the level of OPN and α3β1 integrin proteins while pioglitazone was more effective. Avicenna Research Institute 2020 /pmc/articles/PMC7253937/ /pubmed/32500011 Text en Copyright© 2020, Avicenna Research Institute. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Zarrin, Yasaman
Bakhteyari, Abbas
Nikpour, Parvaneh
Mostafavi, Fatemeh Sadat
Eskandari, Nahid
Matinfar, Mohammad
Aboutorabi, Roshanak
A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
title A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
title_full A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
title_fullStr A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
title_full_unstemmed A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
title_short A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
title_sort study on the presence of osteopontin and α3β1 integrin in the endometrium of diabetic rats at the time of embryo implantation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253937/
https://www.ncbi.nlm.nih.gov/pubmed/32500011
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