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A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation
BACKGROUND: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time o...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Avicenna Research Institute
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253937/ https://www.ncbi.nlm.nih.gov/pubmed/32500011 |
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author | Zarrin, Yasaman Bakhteyari, Abbas Nikpour, Parvaneh Mostafavi, Fatemeh Sadat Eskandari, Nahid Matinfar, Mohammad Aboutorabi, Roshanak |
author_facet | Zarrin, Yasaman Bakhteyari, Abbas Nikpour, Parvaneh Mostafavi, Fatemeh Sadat Eskandari, Nahid Matinfar, Mohammad Aboutorabi, Roshanak |
author_sort | Zarrin, Yasaman |
collection | PubMed |
description | BACKGROUND: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time of endometrial receptivity. METHODS: Twenty-eight female rats were divided into control, diabetic, pioglitazone-treated and metformin-treated groups. Western blot was performed to determine the OPN and α3β1 integrin proteins in rats’ endometrium at the time of implantation. Data were analyzed by analysis of variance (ANOVA) and p<0.05 was considered statistically significant. RESULTS: OPN increased significantly in the diabetic group in comparison with control (p<0.001), metformin-treated (p=0.008) and pioglitazone-treated groups (p< 0.001). Furthermore, α3β1 integrin protein level in diabetic group had a significant difference in comparison with that of the control (p<0.001), metformin-treated (p= 0.026) and pioglitazone-treated groups (p<0.001). CONCLUSION: OPN and α3β1 integrin proteins are involved in embryo implantation and their changes in diabetic condition can affect fertility. Treatment with pioglitazone and metformin improved the level of OPN and α3β1 integrin proteins while pioglitazone was more effective. |
format | Online Article Text |
id | pubmed-7253937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Avicenna Research Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-72539372020-06-03 A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation Zarrin, Yasaman Bakhteyari, Abbas Nikpour, Parvaneh Mostafavi, Fatemeh Sadat Eskandari, Nahid Matinfar, Mohammad Aboutorabi, Roshanak J Reprod Infertil Original Article BACKGROUND: Embryo implantation is a critical and multifactorial phenomenon which can be affected by any alteration in molecular micro construction of endometrium. The aim of the current study was to evaluate the effects of diabetes on osteopontin (OPN) and α3β1 integrin proteins level at the time of endometrial receptivity. METHODS: Twenty-eight female rats were divided into control, diabetic, pioglitazone-treated and metformin-treated groups. Western blot was performed to determine the OPN and α3β1 integrin proteins in rats’ endometrium at the time of implantation. Data were analyzed by analysis of variance (ANOVA) and p<0.05 was considered statistically significant. RESULTS: OPN increased significantly in the diabetic group in comparison with control (p<0.001), metformin-treated (p=0.008) and pioglitazone-treated groups (p< 0.001). Furthermore, α3β1 integrin protein level in diabetic group had a significant difference in comparison with that of the control (p<0.001), metformin-treated (p= 0.026) and pioglitazone-treated groups (p<0.001). CONCLUSION: OPN and α3β1 integrin proteins are involved in embryo implantation and their changes in diabetic condition can affect fertility. Treatment with pioglitazone and metformin improved the level of OPN and α3β1 integrin proteins while pioglitazone was more effective. Avicenna Research Institute 2020 /pmc/articles/PMC7253937/ /pubmed/32500011 Text en Copyright© 2020, Avicenna Research Institute. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Zarrin, Yasaman Bakhteyari, Abbas Nikpour, Parvaneh Mostafavi, Fatemeh Sadat Eskandari, Nahid Matinfar, Mohammad Aboutorabi, Roshanak A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation |
title | A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation |
title_full | A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation |
title_fullStr | A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation |
title_full_unstemmed | A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation |
title_short | A Study on the Presence of Osteopontin and α3β1 Integrin in the Endometrium of Diabetic Rats at the Time of Embryo Implantation |
title_sort | study on the presence of osteopontin and α3β1 integrin in the endometrium of diabetic rats at the time of embryo implantation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253937/ https://www.ncbi.nlm.nih.gov/pubmed/32500011 |
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