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Gut microbiome composition and risk factors in a large cross-sectional IBS cohort

OBJECTIVE: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is difficult to diagnose and treat due to its inherent heterogeneity and unclear aetiology. Although there is evidence suggesting the importance of the microbiome in IBS, this association remains poorly defined. In...

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Autores principales: Agnello, Melissa, Carroll, Lauren N, Imam, Nabeel, Pino, Rodolfo, Palmer, Christina, Varas, Ignacio, Greene, Claire, Hitschfeld, Maureen, Gupta, Sarah, Almonacid, Daniel E, Hoaglin, Michael C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254124/
https://www.ncbi.nlm.nih.gov/pubmed/32518661
http://dx.doi.org/10.1136/bmjgast-2019-000345
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author Agnello, Melissa
Carroll, Lauren N
Imam, Nabeel
Pino, Rodolfo
Palmer, Christina
Varas, Ignacio
Greene, Claire
Hitschfeld, Maureen
Gupta, Sarah
Almonacid, Daniel E
Hoaglin, Michael C
author_facet Agnello, Melissa
Carroll, Lauren N
Imam, Nabeel
Pino, Rodolfo
Palmer, Christina
Varas, Ignacio
Greene, Claire
Hitschfeld, Maureen
Gupta, Sarah
Almonacid, Daniel E
Hoaglin, Michael C
author_sort Agnello, Melissa
collection PubMed
description OBJECTIVE: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is difficult to diagnose and treat due to its inherent heterogeneity and unclear aetiology. Although there is evidence suggesting the importance of the microbiome in IBS, this association remains poorly defined. In the current study, we aimed to characterise a large cross-sectional cohort of patients with self-reported IBS in terms of microbiome composition, demographics, and risk factors. DESIGN: Individuals who had previously submitted a stool sample for 16S microbiome sequencing were sent a comprehensive survey regarding IBS diagnosis, demographics, health history, comorbidities, family history, and symptoms. Log ratio-transformed abundances of microbial taxa were compared between individuals reporting a diagnosis of IBS without any comorbidities and individuals reporting no health conditions. Univariable testing was followed by a multivariable logistic regression model controlling for relevant confounders. RESULTS: Out of 6386 respondents, 1692 reported a diagnosis of IBS without comorbidities and 1124 reported no health conditions. We identified 3 phyla, 15 genera, and 19 species as significantly associated with IBS after adjustment for confounding factors. Demographic risk factors include a family history of gut disorders and reported use of antibiotics in the last year. CONCLUSION: The results of this study confirm important IBS risk factors in a large cohort and support a connection for microbiome compositional changes in IBS pathogenesis. The results also suggest clinical relevance in monitoring and investigating the microbiome in patients with IBS. Further, the exploratory models described here provide a foundation for future studies.
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spelling pubmed-72541242020-06-08 Gut microbiome composition and risk factors in a large cross-sectional IBS cohort Agnello, Melissa Carroll, Lauren N Imam, Nabeel Pino, Rodolfo Palmer, Christina Varas, Ignacio Greene, Claire Hitschfeld, Maureen Gupta, Sarah Almonacid, Daniel E Hoaglin, Michael C BMJ Open Gastroenterol Irritable Bowel Syndrome OBJECTIVE: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder that is difficult to diagnose and treat due to its inherent heterogeneity and unclear aetiology. Although there is evidence suggesting the importance of the microbiome in IBS, this association remains poorly defined. In the current study, we aimed to characterise a large cross-sectional cohort of patients with self-reported IBS in terms of microbiome composition, demographics, and risk factors. DESIGN: Individuals who had previously submitted a stool sample for 16S microbiome sequencing were sent a comprehensive survey regarding IBS diagnosis, demographics, health history, comorbidities, family history, and symptoms. Log ratio-transformed abundances of microbial taxa were compared between individuals reporting a diagnosis of IBS without any comorbidities and individuals reporting no health conditions. Univariable testing was followed by a multivariable logistic regression model controlling for relevant confounders. RESULTS: Out of 6386 respondents, 1692 reported a diagnosis of IBS without comorbidities and 1124 reported no health conditions. We identified 3 phyla, 15 genera, and 19 species as significantly associated with IBS after adjustment for confounding factors. Demographic risk factors include a family history of gut disorders and reported use of antibiotics in the last year. CONCLUSION: The results of this study confirm important IBS risk factors in a large cohort and support a connection for microbiome compositional changes in IBS pathogenesis. The results also suggest clinical relevance in monitoring and investigating the microbiome in patients with IBS. Further, the exploratory models described here provide a foundation for future studies. BMJ Publishing Group 2020-04-06 /pmc/articles/PMC7254124/ /pubmed/32518661 http://dx.doi.org/10.1136/bmjgast-2019-000345 Text en © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Irritable Bowel Syndrome
Agnello, Melissa
Carroll, Lauren N
Imam, Nabeel
Pino, Rodolfo
Palmer, Christina
Varas, Ignacio
Greene, Claire
Hitschfeld, Maureen
Gupta, Sarah
Almonacid, Daniel E
Hoaglin, Michael C
Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
title Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
title_full Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
title_fullStr Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
title_full_unstemmed Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
title_short Gut microbiome composition and risk factors in a large cross-sectional IBS cohort
title_sort gut microbiome composition and risk factors in a large cross-sectional ibs cohort
topic Irritable Bowel Syndrome
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254124/
https://www.ncbi.nlm.nih.gov/pubmed/32518661
http://dx.doi.org/10.1136/bmjgast-2019-000345
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