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CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse

BACKGROUND: The CYP19A1 gene, which encodes the enzyme responsible for androgen aromatization into estrogens, may play an important role in breast cancer aggressiveness. However, no study has evaluated CYP19A1 gene expression in the peripheral blood of women with relapsed breast cancer. METHODS: In...

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Autores principales: Barros-Oliveira, Maria da Conceição, Costa-Silva, Danylo Rafhael, Campos-Verdes, Larysse Cardoso, Pereira, Renato de Oliveira, Silva, Rozirene Araújo, Moura-Borges, Paulo de Tarso, Sousa, Emerson Brandão, Pinho-Sobral, André Luiz, Lopes-Costa, Pedro Vitor, dos Santos, Alesse Ribeiro, Soares-Lopes, Ione Maria Ribeiro, Viana, Jackeline Lopes, Melo, Mariella de Almeida, Manes Neto, Fidelis, Coelho, Eid Gonçalves, Pires e Cruz, Maria do Socorro, Costa-Silva, Vladimir, Gebrim, Luiz Henrique, Da Silva, Benedito Borges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254631/
https://www.ncbi.nlm.nih.gov/pubmed/32460723
http://dx.doi.org/10.1186/s12885-020-06978-z
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author Barros-Oliveira, Maria da Conceição
Costa-Silva, Danylo Rafhael
Campos-Verdes, Larysse Cardoso
Pereira, Renato de Oliveira
Silva, Rozirene Araújo
Moura-Borges, Paulo de Tarso
Sousa, Emerson Brandão
Pinho-Sobral, André Luiz
Lopes-Costa, Pedro Vitor
dos Santos, Alesse Ribeiro
Soares-Lopes, Ione Maria Ribeiro
Viana, Jackeline Lopes
Melo, Mariella de Almeida
Manes Neto, Fidelis
Coelho, Eid Gonçalves
Pires e Cruz, Maria do Socorro
Costa-Silva, Vladimir
Gebrim, Luiz Henrique
Da Silva, Benedito Borges
author_facet Barros-Oliveira, Maria da Conceição
Costa-Silva, Danylo Rafhael
Campos-Verdes, Larysse Cardoso
Pereira, Renato de Oliveira
Silva, Rozirene Araújo
Moura-Borges, Paulo de Tarso
Sousa, Emerson Brandão
Pinho-Sobral, André Luiz
Lopes-Costa, Pedro Vitor
dos Santos, Alesse Ribeiro
Soares-Lopes, Ione Maria Ribeiro
Viana, Jackeline Lopes
Melo, Mariella de Almeida
Manes Neto, Fidelis
Coelho, Eid Gonçalves
Pires e Cruz, Maria do Socorro
Costa-Silva, Vladimir
Gebrim, Luiz Henrique
Da Silva, Benedito Borges
author_sort Barros-Oliveira, Maria da Conceição
collection PubMed
description BACKGROUND: The CYP19A1 gene, which encodes the enzyme responsible for androgen aromatization into estrogens, may play an important role in breast cancer aggressiveness. However, no study has evaluated CYP19A1 gene expression in the peripheral blood of women with relapsed breast cancer. METHODS: In this cross-sectional study, CYP19A1 gene expression was quantified by RT-PCR in the peripheral blood of 146 women with breast cancer who were first divided into two groups according to the expression of CYP19A1 (low and high); each group had 73 patients. Subsequently, women were divided into two groups: those without recurrence (control, n = 85) and those with recurrence (study, n = 61). Statistical analysis of the data was performed using ANOVA, the Mann-Whitney, Chi-square or Fisher’s exact test (p <  0.05). RESULTS: There were no significant differences between the relative expression of CYP19A1 mRNA in the low expression group and the high expression group according to the variables studied. There were no significant differences in CYP19A1 gene expression in the study and control groups (p = 0.8461). In the relapse group, CYP19A1 gene expression was significantly higher in the hybrid luminal subtype than in the triple-negative subtype (p = 0.0321), whereas it was significantly lower in HER2-negative cases than in HER2-positive cases (p <  0.0376). Women with locoregional recurrence showed higher expression than women with distant recurrence (p <  0.0001). CONCLUSIONS: The present study found no significant differences between women with high and low expression of the CYP19A1 gene mRNA or between those in the study group and the control group. However, in women with recurrence, there was increased expression of CYP19A1 mRNA in those who had the luminal hybrid subtype and locoregional relapse and decreased expression in those negative for HER2.
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spelling pubmed-72546312020-06-07 CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse Barros-Oliveira, Maria da Conceição Costa-Silva, Danylo Rafhael Campos-Verdes, Larysse Cardoso Pereira, Renato de Oliveira Silva, Rozirene Araújo Moura-Borges, Paulo de Tarso Sousa, Emerson Brandão Pinho-Sobral, André Luiz Lopes-Costa, Pedro Vitor dos Santos, Alesse Ribeiro Soares-Lopes, Ione Maria Ribeiro Viana, Jackeline Lopes Melo, Mariella de Almeida Manes Neto, Fidelis Coelho, Eid Gonçalves Pires e Cruz, Maria do Socorro Costa-Silva, Vladimir Gebrim, Luiz Henrique Da Silva, Benedito Borges BMC Cancer Research Article BACKGROUND: The CYP19A1 gene, which encodes the enzyme responsible for androgen aromatization into estrogens, may play an important role in breast cancer aggressiveness. However, no study has evaluated CYP19A1 gene expression in the peripheral blood of women with relapsed breast cancer. METHODS: In this cross-sectional study, CYP19A1 gene expression was quantified by RT-PCR in the peripheral blood of 146 women with breast cancer who were first divided into two groups according to the expression of CYP19A1 (low and high); each group had 73 patients. Subsequently, women were divided into two groups: those without recurrence (control, n = 85) and those with recurrence (study, n = 61). Statistical analysis of the data was performed using ANOVA, the Mann-Whitney, Chi-square or Fisher’s exact test (p <  0.05). RESULTS: There were no significant differences between the relative expression of CYP19A1 mRNA in the low expression group and the high expression group according to the variables studied. There were no significant differences in CYP19A1 gene expression in the study and control groups (p = 0.8461). In the relapse group, CYP19A1 gene expression was significantly higher in the hybrid luminal subtype than in the triple-negative subtype (p = 0.0321), whereas it was significantly lower in HER2-negative cases than in HER2-positive cases (p <  0.0376). Women with locoregional recurrence showed higher expression than women with distant recurrence (p <  0.0001). CONCLUSIONS: The present study found no significant differences between women with high and low expression of the CYP19A1 gene mRNA or between those in the study group and the control group. However, in women with recurrence, there was increased expression of CYP19A1 mRNA in those who had the luminal hybrid subtype and locoregional relapse and decreased expression in those negative for HER2. BioMed Central 2020-05-27 /pmc/articles/PMC7254631/ /pubmed/32460723 http://dx.doi.org/10.1186/s12885-020-06978-z Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Barros-Oliveira, Maria da Conceição
Costa-Silva, Danylo Rafhael
Campos-Verdes, Larysse Cardoso
Pereira, Renato de Oliveira
Silva, Rozirene Araújo
Moura-Borges, Paulo de Tarso
Sousa, Emerson Brandão
Pinho-Sobral, André Luiz
Lopes-Costa, Pedro Vitor
dos Santos, Alesse Ribeiro
Soares-Lopes, Ione Maria Ribeiro
Viana, Jackeline Lopes
Melo, Mariella de Almeida
Manes Neto, Fidelis
Coelho, Eid Gonçalves
Pires e Cruz, Maria do Socorro
Costa-Silva, Vladimir
Gebrim, Luiz Henrique
Da Silva, Benedito Borges
CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse
title CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse
title_full CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse
title_fullStr CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse
title_full_unstemmed CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse
title_short CYP19A1 gene expression in the peripheral blood of Brazilian women with breast cancer relapse
title_sort cyp19a1 gene expression in the peripheral blood of brazilian women with breast cancer relapse
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254631/
https://www.ncbi.nlm.nih.gov/pubmed/32460723
http://dx.doi.org/10.1186/s12885-020-06978-z
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