Irregularly Shaped Iron Nitride Nanoparticles as a Potential Candidate for Biomedical Applications: From Synthesis to Characterization

[Image: see text] Magnetic nanoparticles (MNPs) have been extensively used in drug/gene delivery, hyperthermia therapy, magnetic particle imaging (MPI), magnetic resonance imaging (MRI), magnetic bioassays, and so forth. With proper surface chemical modifications, physicochemically stable and nontoxic MNPs are emerging contrast agents and tracers for in vivo MRI and MPI applications. Herein, we report the high magnetic moment, irregularly shaped γ′-Fe(4)N nanoparticles for enhanced hyperthermia therapy and T(2) contrast agent for MRI application. The static and dynamic magnetic properties of γ′-Fe(4)N nanoparticles are characterized by a vibrating sample magnetometer (VSM) and a magnetic particle spectroscopy (MPS) system, respectively. Compared to the γ-Fe(2)O(3) nanoparticles, γ′-Fe(4)N nanoparticles show at least three times higher saturation magnetization, which, as a result, gives rise to the stronger dynamic magnetic responses as proved in the MPS measurement results. In addition, γ′-Fe(4)N nanoparticles are functionalized with an oleic acid layer by a wet mechanical milling process. The morphologies of as-milled nanoparticles are characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), and nanoparticle tracking analyzer (NTA). We report that with proper surface chemical modification and tuning on morphologies, γ′-Fe(4)N nanoparticles could be used as tiny heating sources for hyperthermia and contrast agents for MRI applications with minimum dose.