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miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma
Emerging evidence has indicated that microRNAs (miRs) are involved in the malignant behavior of cancer. The present study explored the role of miR-193b in the development and metastasis of osteosarcoma. Compared with F4 osteosarcoma cells, which have a relatively low metastatic potential, highly met...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254955/ https://www.ncbi.nlm.nih.gov/pubmed/32377743 http://dx.doi.org/10.3892/or.2020.7601 |
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author | Gao, Jinjian Ma, Sai Yang, Fan Chen, Xu Wang, Wei Zhang, Jianping Li, Yufang Wang, Tao Shan, Lequn |
author_facet | Gao, Jinjian Ma, Sai Yang, Fan Chen, Xu Wang, Wei Zhang, Jianping Li, Yufang Wang, Tao Shan, Lequn |
author_sort | Gao, Jinjian |
collection | PubMed |
description | Emerging evidence has indicated that microRNAs (miRs) are involved in the malignant behavior of cancer. The present study explored the role of miR-193b in the development and metastasis of osteosarcoma. Compared with F4 osteosarcoma cells, which have a relatively low metastatic potential, highly metastatic F5M2 cells exhibited a lower expression of miR-193b. Furthermore, miR-193b exerted negative effects on cell proliferation, colony formation, cell cycle progression, migration and invasion, and induced apoptosis. In vivo studies revealed negative influences of miR-193b on tumorigenesis and metastasis. The tumor-suppressive role of miR-193b was achieved by targeting KRAS and stathmin 1 (STMN1). Notably, overexpression of KRAS and STMN1 attenuated the miR-193b-induced inhibition of malignant behaviors. There was a double-negative regulatory loop between MYC and miR-193b, with MYC inhibiting miR-193b expression by directly binding to its promoter region and miR-193b negatively influencing MYC expression indirectly through some unknown mechanism. Collectively, these findings indicated that miR-193b may serve a tumor suppressive role in osteosarcoma by targeting KRAS and STMN1. The double-negative regulatory loop between MYC and miR-193b may contribute to the sustained upregulation of MYC, the downregulation of miR-193b, and to the subsequently enhanced expression of KRAS and STMN1, which may eventually lead to the development and metastasis of osteosarcoma. |
format | Online Article Text |
id | pubmed-7254955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-72549552020-05-31 miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma Gao, Jinjian Ma, Sai Yang, Fan Chen, Xu Wang, Wei Zhang, Jianping Li, Yufang Wang, Tao Shan, Lequn Oncol Rep Articles Emerging evidence has indicated that microRNAs (miRs) are involved in the malignant behavior of cancer. The present study explored the role of miR-193b in the development and metastasis of osteosarcoma. Compared with F4 osteosarcoma cells, which have a relatively low metastatic potential, highly metastatic F5M2 cells exhibited a lower expression of miR-193b. Furthermore, miR-193b exerted negative effects on cell proliferation, colony formation, cell cycle progression, migration and invasion, and induced apoptosis. In vivo studies revealed negative influences of miR-193b on tumorigenesis and metastasis. The tumor-suppressive role of miR-193b was achieved by targeting KRAS and stathmin 1 (STMN1). Notably, overexpression of KRAS and STMN1 attenuated the miR-193b-induced inhibition of malignant behaviors. There was a double-negative regulatory loop between MYC and miR-193b, with MYC inhibiting miR-193b expression by directly binding to its promoter region and miR-193b negatively influencing MYC expression indirectly through some unknown mechanism. Collectively, these findings indicated that miR-193b may serve a tumor suppressive role in osteosarcoma by targeting KRAS and STMN1. The double-negative regulatory loop between MYC and miR-193b may contribute to the sustained upregulation of MYC, the downregulation of miR-193b, and to the subsequently enhanced expression of KRAS and STMN1, which may eventually lead to the development and metastasis of osteosarcoma. D.A. Spandidos 2020-07 2020-04-29 /pmc/articles/PMC7254955/ /pubmed/32377743 http://dx.doi.org/10.3892/or.2020.7601 Text en Copyright: © Gao et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Gao, Jinjian Ma, Sai Yang, Fan Chen, Xu Wang, Wei Zhang, Jianping Li, Yufang Wang, Tao Shan, Lequn miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma |
title | miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma |
title_full | miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma |
title_fullStr | miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma |
title_full_unstemmed | miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma |
title_short | miR-193b exhibits mutual interaction with MYC, and suppresses growth and metastasis of osteosarcoma |
title_sort | mir-193b exhibits mutual interaction with myc, and suppresses growth and metastasis of osteosarcoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254955/ https://www.ncbi.nlm.nih.gov/pubmed/32377743 http://dx.doi.org/10.3892/or.2020.7601 |
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