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Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration
Rationale: The effectiveness of stem cell based-therapy for bone regeneration has been demonstrated; yet, clinical application of autologous stem cells is still limited by invasive acquisition, long culture processes, and high cost. Besides, it remains controversial whether autologous stem cells cou...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255030/ https://www.ncbi.nlm.nih.gov/pubmed/32483449 http://dx.doi.org/10.7150/thno.45249 |
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author | Jiang, Fei Zhang, Wenjie Zhou, Mingliang Zhou, Zhixuan Shen, Ming Chen, Ning Jiang, Xinquan |
author_facet | Jiang, Fei Zhang, Wenjie Zhou, Mingliang Zhou, Zhixuan Shen, Ming Chen, Ning Jiang, Xinquan |
author_sort | Jiang, Fei |
collection | PubMed |
description | Rationale: The effectiveness of stem cell based-therapy for bone regeneration has been demonstrated; yet, clinical application of autologous stem cells is still limited by invasive acquisition, long culture processes, and high cost. Besides, it remains controversial whether autologous stem cells could directly participate in tissue repair after differentiation. Thus, increasing allogeneic stem cells have been developed into drugs to indirectly activate endogenous regeneration and induce tissue regeneration. Human amniotic mesenchymal stromal cells (HAMSCs) have been extensively studied, showing multiple regulatory functions, but mechanisms of HAMSCs in promoting bone regeneration are remain unclear. Methods: Proteome profile of HAMSCs and their functions on vascularized bone regeneration were investigated in vitro, while rabbit cranial defect model was used to further detect the effects of bone formation in vivo. Results: HAMSCs secrete many osteogenic, angiogenic, and immunomodulatory cytokines. In vitro, HAMSCs can promote human bone-marrow mesenchymal stromal cells (HBMSCs) migration and osteogenic differentiation; promote the capillary-tube formation of human umbilical vascular endothelial cells (HUVECs), induce HUVECs migration and pro-angiogenic genes expression, and promote M2 macrophage polarization. Further, in vivo studies suggested that transplanted HAMSCs could survive and induce M2 macrophages to secrete bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) in rabbits' skull defects at an early stage, and, in turn, promote more new bone formation. Conclusion: HAMSCs have good biocompatibility and paracrine function to promote bone repair by stimulating endogenous regeneration. |
format | Online Article Text |
id | pubmed-7255030 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-72550302020-05-31 Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration Jiang, Fei Zhang, Wenjie Zhou, Mingliang Zhou, Zhixuan Shen, Ming Chen, Ning Jiang, Xinquan Theranostics Research Paper Rationale: The effectiveness of stem cell based-therapy for bone regeneration has been demonstrated; yet, clinical application of autologous stem cells is still limited by invasive acquisition, long culture processes, and high cost. Besides, it remains controversial whether autologous stem cells could directly participate in tissue repair after differentiation. Thus, increasing allogeneic stem cells have been developed into drugs to indirectly activate endogenous regeneration and induce tissue regeneration. Human amniotic mesenchymal stromal cells (HAMSCs) have been extensively studied, showing multiple regulatory functions, but mechanisms of HAMSCs in promoting bone regeneration are remain unclear. Methods: Proteome profile of HAMSCs and their functions on vascularized bone regeneration were investigated in vitro, while rabbit cranial defect model was used to further detect the effects of bone formation in vivo. Results: HAMSCs secrete many osteogenic, angiogenic, and immunomodulatory cytokines. In vitro, HAMSCs can promote human bone-marrow mesenchymal stromal cells (HBMSCs) migration and osteogenic differentiation; promote the capillary-tube formation of human umbilical vascular endothelial cells (HUVECs), induce HUVECs migration and pro-angiogenic genes expression, and promote M2 macrophage polarization. Further, in vivo studies suggested that transplanted HAMSCs could survive and induce M2 macrophages to secrete bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) in rabbits' skull defects at an early stage, and, in turn, promote more new bone formation. Conclusion: HAMSCs have good biocompatibility and paracrine function to promote bone repair by stimulating endogenous regeneration. Ivyspring International Publisher 2020-05-15 /pmc/articles/PMC7255030/ /pubmed/32483449 http://dx.doi.org/10.7150/thno.45249 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Jiang, Fei Zhang, Wenjie Zhou, Mingliang Zhou, Zhixuan Shen, Ming Chen, Ning Jiang, Xinquan Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
title | Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
title_full | Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
title_fullStr | Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
title_full_unstemmed | Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
title_short | Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
title_sort | human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255030/ https://www.ncbi.nlm.nih.gov/pubmed/32483449 http://dx.doi.org/10.7150/thno.45249 |
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