Cargando…

Drosophila balancer reengineering using polycistronic gRNA for CRISPR/Cas9 gene editing

Detalles Bibliográficos
Autores principales:	Benner, Leif, Oliver, Brian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2018
Materias:	Materials and Reagents
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255806/ https://www.ncbi.nlm.nih.gov/pubmed/32550371 http://dx.doi.org/10.17912/sg7d-sd61

Ejemplares similares

Genome editing of Caenorhabditis briggsae using CRISPR/Cas9 co-conversion marker dpy-10
por: Cohen, Sarah M, et al.
Publicado: (2019)

The Cbr-DPY-10(Arg92Cys) modification is a reliable co-conversion marker for CRISPR/Cas9 genome editing in Caenorhabditis briggsae
por: Toker, Itai Antoine, et al.
Publicado: (2022)

Optimization of the CRISPR/Cas9 system using adh1 promoter derivatives in fission yeast
por: Saito, Miori, et al.
Publicado: (2023)

Efficient production of CRISPR/Cas9 gene knockouts in the male/female nematode Caenorhabditis nigoni
por: Harbin, Jonathan P, et al.
Publicado: (2023)

mScarlet and split fluorophore mScarlet resources for plasmid-based CRISPR/Cas9 knock-in in C. elegans
por: Witten, Gillian, et al.
Publicado: (2023)

Extending the CRISPR toolbox for C. elegans: Cpf1 as an alternative gene editing system for AT-rich sequences
por: Ebbing, Annabel, et al.
Publicado: (2017)

IgG antibodies label the spermatozoan centriole of Drosophila melanogaster
por: Jaiswal, Ankit, et al.
Publicado: (2023)

Creation of a functional unc-11/PICALM GFP knock-in by CRISPR
por: Gallegos, Maria, et al.
Publicado: (2021)

Near-infrared nuclear markers for Drosophila imaging
por: Pan, Yuanwang, et al.
Publicado: (2022)

Balancer-assisted outcrossing to remove unwanted background mutations
por: Dejima, Katsufumi, et al.
Publicado: (2022)

A marker-free genome editing method in S. pombe using the delitto perfetto approach.
por: Fréon, Karine, et al.
Publicado: (2023)

Serum-free adapted Drosophila S2R+ line is amenable to RNA interference
por: Luhur, Arthur, et al.
Publicado: (2021)

Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila
por: Takemura, Masahiko, et al.
Publicado: (2021)

Generation and validation of pX-UASTattB for dose-dependent misexpression studies in Drosophila
por: Singh, Monika, et al.
Publicado: (2022)

Enteroendocrine cell expression of split-GAL4 drivers bearing regulatory sequences associated with panneuronally expressed genes in Drosophila melanogaster
por: Holsopple, Jessica M., et al.
Publicado: (2022)

Comparison of neuronal GAL4 drivers along with the AGES (auxin-inducible gene expression system) and TARGET (temporal and regional gene expression targeting) systems for fine tuning of neuronal gene expression in Drosophila
por: Hawley, Hannah R, et al.
Publicado: (2023)

A new anterior pharyngeal region specific fluorescent co-transformation marker
por: Bhattacharya, Abhishek, et al.
Publicado: (2019)

An antibody staining protocol variation for nematodes that adds heat-induced antigen retrieval (HIAR)
por: Loer, Curtis, et al.
Publicado: (2019)

gRNA validation for wheat genome editing with the CRISPR-Cas9 system
por: Arndell, Taj, et al.
Publicado: (2019)

Expression of a FRET-based ATP Biosensor in the C. elegans Intestine
por: Soto, Jose, et al.
Publicado: (2020)

Immunofluorescence reveals neuron-specific promoter activity in non-neuronal cells
por: Lear, Sierra K., et al.
Publicado: (2018)

Worm corpses affect quantification of dauer recovery
por: Gabaldon, Carolaing, et al.
Publicado: (2019)

Using HDR and a template to introduce an in-frame HA tag on the 3′ end of the Xenopus laevis gata2.L open reading frame
por: Piccinni, Maya, et al.
Publicado: (2019)

Phase-separated protein dynamics are affected by fluorescent tag choice
por: Uebel, Celja J, et al.
Publicado: (2019)

Modified auxin improves the auxin-inducible degradation (AID) system for laid C. elegans embryos
por: Negishi, Takefumi, et al.
Publicado: (2019)

Crossing two sperm chromatin-localized mCherry transgenes into a single C. elegans strain boosts signal intensity without harming sperm function
por: Wong, Amanda C, et al.
Publicado: (2020)

Tracking of centriole inheritance in C. elegans
por: Erpf, Anna C., et al.
Publicado: (2020)

DVC interneuron cGAL driver in Caenorhabditis elegans
por: Oh, Jun Young, et al.
Publicado: (2019)

Simplified detection of a point mutation in C. elegans using tetra-primer ARMS-PCR
por: Sullenberger, Matthew T., et al.
Publicado: (2018)

Mapping results for a set of cGAL effectors and drivers
por: Walton, Sophie J., et al.
Publicado: (2017)

Auxin does not affect a suite of morphological or behavioral phenotypes in two wild-type C. elegans strains
por: McDiarmid, Troy A, et al.
Publicado: (2020)

Actin polymerization is not required for the fast block to polyspermy in the African clawed frog, Xenopus laevis
por: Tembo, Maiwase, et al.
Publicado: (2021)

Endogenous expression and localization of HIS-72::mTurquoise2 in C. elegans
por: Sloan, Dillon E., et al.
Publicado: (2021)

Transgenic reporter analysis of ChIP-Seq-defined enhancers identifies novel target genes for the terminal selector UNC-3/Collier/Ebf
por: Li, Yinan, et al.
Publicado: (2021)

Improved GAL4 and Tet OFF drivers for C. elegans bipartite expression
por: Nonet, Michael
Publicado: (2021)

The proline-rich domain of MML-1 is biologically important but not required for localization to target promoters
por: Ceballos, Ainhoa, et al.
Publicado: (2021)

Efficient generation of a single-copy eft-3p::TIR1::F2A:: BFP::AID*::NLS allele in the C. elegans ttTi5605 insertion site through recombination-mediated cassette exchange
por: Vo, An A., et al.
Publicado: (2021)

Visualization of a curated Oryza sativa L. CDPKs Protein-Protein Interaction Network (CDPK-OsPPIN)
por: Marques, Joana, et al.
Publicado: (2022)

Optimizing expression of a single copy transgene in C. elegans
por: Dour, Scott, et al.
Publicado: (2021)

A histamine-gated channel is an efficient negative selection marker for C. elegans transgenesis
por: El Mouridi, Sonia, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_s6l9t036vf1fnmk40g6uuet26i