Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with...

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Detalles Bibliográficos
Autores principales: Grant, Stephen D., Cairns, Gemma S., Wistuba, Jordan, Patton, Brian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255852/
https://www.ncbi.nlm.nih.gov/pubmed/32518624
http://dx.doi.org/10.12688/f1000research.21294.1
Descripción
Sumario:We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.

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