Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging

We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with...

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Detalles Bibliográficos
Autores principales: Grant, Stephen D., Cairns, Gemma S., Wistuba, Jordan, Patton, Brian R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255852/
https://www.ncbi.nlm.nih.gov/pubmed/32518624
http://dx.doi.org/10.12688/f1000research.21294.1
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author Grant, Stephen D.
Cairns, Gemma S.
Wistuba, Jordan
Patton, Brian R.
author_facet Grant, Stephen D.
Cairns, Gemma S.
Wistuba, Jordan
Patton, Brian R.
author_sort Grant, Stephen D.
collection PubMed
description We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200.
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spelling pubmed-72558522020-06-08 Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging Grant, Stephen D. Cairns, Gemma S. Wistuba, Jordan Patton, Brian R. F1000Res Method Article We report on a 3D printed microscope, based on a design by the Openflexure project, that uses low cost components to perform fluorescence imaging. The system is sufficiently sensitive and mechanically stable to allow the use of the Super Resolution Radial Fluctuations algorithm to obtain images with resolution better than the diffraction limit. Due to the low-cost components, the entire system can be built for approximately $1200. F1000 Research Limited 2019-11-26 /pmc/articles/PMC7255852/ /pubmed/32518624 http://dx.doi.org/10.12688/f1000research.21294.1 Text en Copyright: © 2019 Grant SD et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method Article
Grant, Stephen D.
Cairns, Gemma S.
Wistuba, Jordan
Patton, Brian R.
Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging
title Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging
title_full Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging
title_fullStr Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging
title_full_unstemmed Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging
title_short Adapting the 3D-printed Openflexure microscope enables computational super-resolution imaging
title_sort adapting the 3d-printed openflexure microscope enables computational super-resolution imaging
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7255852/
https://www.ncbi.nlm.nih.gov/pubmed/32518624
http://dx.doi.org/10.12688/f1000research.21294.1
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AT wistubajordan adaptingthe3dprintedopenflexuremicroscopeenablescomputationalsuperresolutionimaging
AT pattonbrianr adaptingthe3dprintedopenflexuremicroscopeenablescomputationalsuperresolutionimaging

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