Cargando…

Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production

OBJECTIVE: Oxidative stress and ultraviolet B (UVB) irradiation are known as principal inducers of DNA damage and modulators of gene expression in aging process and skin photoaging, which are associated with upregulation of matrix metalloproteinases (MMPs). Because of the antioxidant capacity of juj...

Descripción completa

Detalles Bibliográficos
Autores principales: Abotorabi, Zahra, Khorashadizadeh, Mohsen, Arab, Mina, Hassanpour Fard, Mohammad, Zarban, Asghar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256284/
https://www.ncbi.nlm.nih.gov/pubmed/32523883
_version_ 1783539877956026368
author Abotorabi, Zahra
Khorashadizadeh, Mohsen
Arab, Mina
Hassanpour Fard, Mohammad
Zarban, Asghar
author_facet Abotorabi, Zahra
Khorashadizadeh, Mohsen
Arab, Mina
Hassanpour Fard, Mohammad
Zarban, Asghar
author_sort Abotorabi, Zahra
collection PubMed
description OBJECTIVE: Oxidative stress and ultraviolet B (UVB) irradiation are known as principal inducers of DNA damage and modulators of gene expression in aging process and skin photoaging, which are associated with upregulation of matrix metalloproteinases (MMPs). Because of the antioxidant capacity of jujube and green tea, we decided to determine their protective effects of human fibroblast cells against UVB-induced photo-damage and reduction of MMP-2 and MMP-9 expression. MATERIALS AND METHODS: We exposed human fibroblast cells to different doses of UVB (0-20 mJ/cm(2)) with or without different concentrations of jujube and green tea extracts. Cell viability was assessed using MTT assay. Total antioxidant capacity and free radical scavenging activity of cell supernatant were assessed using FRAP and DPPH methods, respectively. The concentrations of MMP-2 and MMP-9 in the samples were determined by ELISA kits. RESULTS: Fibroblast cells viability, 24 hr after UVB irradiation, reduced about 70% compared to the controls. Pre-treatment of the cells with jujube extract (8 mg/ml) increased the cell viability by almost 85% while green tea (0.5 mg/ml) protected the irradiated cells by 71%. Also, MMP-2 and MMP-9 content decreased in a concentration-dependent manner in the cells pre-treated with jujube and green tea extracts. CONCLUSION: These data suggest that jujube and green tea could be useful to attenuate solar UVB light-induced oxidative stress and skin photoaging and can be suggested as a potential candidate for the development of new anti-UVB medicines and cosmetic products.
format Online
Article
Text
id pubmed-7256284
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-72562842020-06-09 Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production Abotorabi, Zahra Khorashadizadeh, Mohsen Arab, Mina Hassanpour Fard, Mohammad Zarban, Asghar Avicenna J Phytomed Original Research Article OBJECTIVE: Oxidative stress and ultraviolet B (UVB) irradiation are known as principal inducers of DNA damage and modulators of gene expression in aging process and skin photoaging, which are associated with upregulation of matrix metalloproteinases (MMPs). Because of the antioxidant capacity of jujube and green tea, we decided to determine their protective effects of human fibroblast cells against UVB-induced photo-damage and reduction of MMP-2 and MMP-9 expression. MATERIALS AND METHODS: We exposed human fibroblast cells to different doses of UVB (0-20 mJ/cm(2)) with or without different concentrations of jujube and green tea extracts. Cell viability was assessed using MTT assay. Total antioxidant capacity and free radical scavenging activity of cell supernatant were assessed using FRAP and DPPH methods, respectively. The concentrations of MMP-2 and MMP-9 in the samples were determined by ELISA kits. RESULTS: Fibroblast cells viability, 24 hr after UVB irradiation, reduced about 70% compared to the controls. Pre-treatment of the cells with jujube extract (8 mg/ml) increased the cell viability by almost 85% while green tea (0.5 mg/ml) protected the irradiated cells by 71%. Also, MMP-2 and MMP-9 content decreased in a concentration-dependent manner in the cells pre-treated with jujube and green tea extracts. CONCLUSION: These data suggest that jujube and green tea could be useful to attenuate solar UVB light-induced oxidative stress and skin photoaging and can be suggested as a potential candidate for the development of new anti-UVB medicines and cosmetic products. Mashhad University of Medical Sciences 2020 /pmc/articles/PMC7256284/ /pubmed/32523883 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Abotorabi, Zahra
Khorashadizadeh, Mohsen
Arab, Mina
Hassanpour Fard, Mohammad
Zarban, Asghar
Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
title Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
title_full Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
title_fullStr Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
title_full_unstemmed Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
title_short Jujube and green tea extracts protect human fibroblast cells against UVB-mediated photo damage and MMP-2 and MMP-9 production
title_sort jujube and green tea extracts protect human fibroblast cells against uvb-mediated photo damage and mmp-2 and mmp-9 production
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256284/
https://www.ncbi.nlm.nih.gov/pubmed/32523883
work_keys_str_mv AT abotorabizahra jujubeandgreenteaextractsprotecthumanfibroblastcellsagainstuvbmediatedphotodamageandmmp2andmmp9production
AT khorashadizadehmohsen jujubeandgreenteaextractsprotecthumanfibroblastcellsagainstuvbmediatedphotodamageandmmp2andmmp9production
AT arabmina jujubeandgreenteaextractsprotecthumanfibroblastcellsagainstuvbmediatedphotodamageandmmp2andmmp9production
AT hassanpourfardmohammad jujubeandgreenteaextractsprotecthumanfibroblastcellsagainstuvbmediatedphotodamageandmmp2andmmp9production
AT zarbanasghar jujubeandgreenteaextractsprotecthumanfibroblastcellsagainstuvbmediatedphotodamageandmmp2andmmp9production