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lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation

Glioma is a brain cancer characterized by strong invasiveness with limited treatment options and poor prognosis. Recently, dysregulation of long non-coding RNAs (lncRNAs) has emerged as an important component in cellular processes and tumorigenesis. In this study, we demonstrated that TATA-box bindi...

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Autores principales: Ruan, Xuelei, Zheng, Jian, Liu, Xiaobai, Liu, Yunhui, Liu, Libo, Ma, Jun, He, Qianru, Yang, Chunqing, Wang, Di, Cai, Heng, Li, Zhen, Liu, Jing, Xue, Yixue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256440/
https://www.ncbi.nlm.nih.gov/pubmed/32464546
http://dx.doi.org/10.1016/j.omtn.2020.05.003
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author Ruan, Xuelei
Zheng, Jian
Liu, Xiaobai
Liu, Yunhui
Liu, Libo
Ma, Jun
He, Qianru
Yang, Chunqing
Wang, Di
Cai, Heng
Li, Zhen
Liu, Jing
Xue, Yixue
author_facet Ruan, Xuelei
Zheng, Jian
Liu, Xiaobai
Liu, Yunhui
Liu, Libo
Ma, Jun
He, Qianru
Yang, Chunqing
Wang, Di
Cai, Heng
Li, Zhen
Liu, Jing
Xue, Yixue
author_sort Ruan, Xuelei
collection PubMed
description Glioma is a brain cancer characterized by strong invasiveness with limited treatment options and poor prognosis. Recently, dysregulation of long non-coding RNAs (lncRNAs) has emerged as an important component in cellular processes and tumorigenesis. In this study, we demonstrated that TATA-box binding protein associated factor 15 (TAF15) and long intergenic non-protein coding RNA 665 (LINC00665) were both downregulated in glioma tissues and cells. TAF15 overexpression enhanced the stability of LINC00665, inhibiting malignant biological behaviors of glioma cells. Both metal regulatory transcription factor 1 (MTF1) and YY2 transcription factor (YY2) showed high expression levels in glioma tissues and cells, and their knockdown inhibited malignant progression. Mechanistically, overexpression of LINC00665 was confirmed to destabilize MTF1 and YY2 mRNA by interacting with STAU1, and knockdown of STAU1 could rescue the MTF1 and YY2 mRNA degradation caused by LINC00665 overexpression. G(2) and S-phase expressed 1 (GTSE1) was identified as an oncogene in glioma, and knockdown of MTF1 or YY2 decreased the mRNA and protein expression levels of GTSE1 through direct binding to the GTSE1 promoter region. Our study highlights a key role of the TAF15/LINC00665/MTF1(YY2)/GTSE1 axis in modulating the malignant biological behaviors of glioma cells, suggesting novel mechanisms by which lncRNAs affect STAU1-mediated mRNA stability, which can inform new molecular therapies for glioma.
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spelling pubmed-72564402020-06-01 lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation Ruan, Xuelei Zheng, Jian Liu, Xiaobai Liu, Yunhui Liu, Libo Ma, Jun He, Qianru Yang, Chunqing Wang, Di Cai, Heng Li, Zhen Liu, Jing Xue, Yixue Mol Ther Nucleic Acids Article Glioma is a brain cancer characterized by strong invasiveness with limited treatment options and poor prognosis. Recently, dysregulation of long non-coding RNAs (lncRNAs) has emerged as an important component in cellular processes and tumorigenesis. In this study, we demonstrated that TATA-box binding protein associated factor 15 (TAF15) and long intergenic non-protein coding RNA 665 (LINC00665) were both downregulated in glioma tissues and cells. TAF15 overexpression enhanced the stability of LINC00665, inhibiting malignant biological behaviors of glioma cells. Both metal regulatory transcription factor 1 (MTF1) and YY2 transcription factor (YY2) showed high expression levels in glioma tissues and cells, and their knockdown inhibited malignant progression. Mechanistically, overexpression of LINC00665 was confirmed to destabilize MTF1 and YY2 mRNA by interacting with STAU1, and knockdown of STAU1 could rescue the MTF1 and YY2 mRNA degradation caused by LINC00665 overexpression. G(2) and S-phase expressed 1 (GTSE1) was identified as an oncogene in glioma, and knockdown of MTF1 or YY2 decreased the mRNA and protein expression levels of GTSE1 through direct binding to the GTSE1 promoter region. Our study highlights a key role of the TAF15/LINC00665/MTF1(YY2)/GTSE1 axis in modulating the malignant biological behaviors of glioma cells, suggesting novel mechanisms by which lncRNAs affect STAU1-mediated mRNA stability, which can inform new molecular therapies for glioma. American Society of Gene & Cell Therapy 2020-05-08 /pmc/articles/PMC7256440/ /pubmed/32464546 http://dx.doi.org/10.1016/j.omtn.2020.05.003 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ruan, Xuelei
Zheng, Jian
Liu, Xiaobai
Liu, Yunhui
Liu, Libo
Ma, Jun
He, Qianru
Yang, Chunqing
Wang, Di
Cai, Heng
Li, Zhen
Liu, Jing
Xue, Yixue
lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation
title lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation
title_full lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation
title_fullStr lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation
title_full_unstemmed lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation
title_short lncRNA LINC00665 Stabilized by TAF15 Impeded the Malignant Biological Behaviors of Glioma Cells via STAU1-Mediated mRNA Degradation
title_sort lncrna linc00665 stabilized by taf15 impeded the malignant biological behaviors of glioma cells via stau1-mediated mrna degradation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256440/
https://www.ncbi.nlm.nih.gov/pubmed/32464546
http://dx.doi.org/10.1016/j.omtn.2020.05.003
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