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A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels

Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) have great potential for regenerative medicine and drug discovery. In this study, we developed a novel protocol to more reproducibly and efficiently induce cardiomyocytes. A large quantity of uniformly sized spheroids w...

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Detalles Bibliográficos
Autores principales: Miwa, Tatsuaki, Idiris, Alimjan, Kumagai, Hiromichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256449/
https://www.ncbi.nlm.nih.gov/pubmed/32490063
http://dx.doi.org/10.1016/j.reth.2020.04.008
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author Miwa, Tatsuaki
Idiris, Alimjan
Kumagai, Hiromichi
author_facet Miwa, Tatsuaki
Idiris, Alimjan
Kumagai, Hiromichi
author_sort Miwa, Tatsuaki
collection PubMed
description Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) have great potential for regenerative medicine and drug discovery. In this study, we developed a novel protocol to more reproducibly and efficiently induce cardiomyocytes. A large quantity of uniformly sized spheroids were generated from hiPSCs using microfabricated vessels and induced into cardiac differentiation. In the middle of the cardiac differentiation process, spheroids were then dissociated into single cells and reaggregated into smaller spheroids using the microfabricated vessels. This reaggregation process raised WNT5A and WNT11 expression levels and improved the quality of cardiomyocyte population compared to that in a control group in which dissociation and reaggregation were not performed.
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spelling pubmed-72564492020-06-01 A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels Miwa, Tatsuaki Idiris, Alimjan Kumagai, Hiromichi Regen Ther Original Article Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) have great potential for regenerative medicine and drug discovery. In this study, we developed a novel protocol to more reproducibly and efficiently induce cardiomyocytes. A large quantity of uniformly sized spheroids were generated from hiPSCs using microfabricated vessels and induced into cardiac differentiation. In the middle of the cardiac differentiation process, spheroids were then dissociated into single cells and reaggregated into smaller spheroids using the microfabricated vessels. This reaggregation process raised WNT5A and WNT11 expression levels and improved the quality of cardiomyocyte population compared to that in a control group in which dissociation and reaggregation were not performed. Japanese Society for Regenerative Medicine 2020-05-26 /pmc/articles/PMC7256449/ /pubmed/32490063 http://dx.doi.org/10.1016/j.reth.2020.04.008 Text en © 2020 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Miwa, Tatsuaki
Idiris, Alimjan
Kumagai, Hiromichi
A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
title A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
title_full A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
title_fullStr A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
title_full_unstemmed A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
title_short A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
title_sort novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256449/
https://www.ncbi.nlm.nih.gov/pubmed/32490063
http://dx.doi.org/10.1016/j.reth.2020.04.008
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