Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52

In vitro studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic Escherichia coli (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory co...

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Autores principales: AL-MNASER, AFNAN A., WOODWARD, MARTIN J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2020
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256741/
https://www.ncbi.nlm.nih.gov/pubmed/32067440
http://dx.doi.org/10.33073/pjm-2020-003
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author AL-MNASER, AFNAN A.
WOODWARD, MARTIN J.
author_facet AL-MNASER, AFNAN A.
WOODWARD, MARTIN J.
author_sort AL-MNASER, AFNAN A.
collection PubMed
description In vitro studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic Escherichia coli (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) to the phytochemicals were selected after growing Escherichia coli (E. coli) strain O23:H52 at sub-lethal concentrations of carvacrol and oregano for a period of 60 days. Whole-genome sequencing (WGS) of two derivatives revealed a missense mutation in cadC and marR: the genes responsible for survival mechanisms and antibiotic resistance by efflux, respectively.
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spelling pubmed-72567412020-06-03 Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52 AL-MNASER, AFNAN A. WOODWARD, MARTIN J. Pol J Microbiol Microbiology In vitro studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic Escherichia coli (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) to the phytochemicals were selected after growing Escherichia coli (E. coli) strain O23:H52 at sub-lethal concentrations of carvacrol and oregano for a period of 60 days. Whole-genome sequencing (WGS) of two derivatives revealed a missense mutation in cadC and marR: the genes responsible for survival mechanisms and antibiotic resistance by efflux, respectively. Exeley Inc. 2020-03 2020-02-18 /pmc/articles/PMC7256741/ /pubmed/32067440 http://dx.doi.org/10.33073/pjm-2020-003 Text en © 2020 Afnan A. Al-Mnaser and Martin J. Woodward https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology
AL-MNASER, AFNAN A.
WOODWARD, MARTIN J.
Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52
title Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52
title_full Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52
title_fullStr Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52
title_full_unstemmed Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52
title_short Sub-lethal Concentrations of Phytochemicals (Carvacrol and Oregano) Select for Reduced Susceptibility Mutants of Escherichia coli O23:H52
title_sort sub-lethal concentrations of phytochemicals (carvacrol and oregano) select for reduced susceptibility mutants of escherichia coli o23:h52
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256741/
https://www.ncbi.nlm.nih.gov/pubmed/32067440
http://dx.doi.org/10.33073/pjm-2020-003
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