Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR

The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic Y. enterocolitica in animal and food samples. In this study, 100 samples of pig tonsils...

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Autor principal: STACHELSKA, MILENA A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256803/
https://www.ncbi.nlm.nih.gov/pubmed/30015460
http://dx.doi.org/10.21307/pjm-2018-023
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author STACHELSKA, MILENA A.
author_facet STACHELSKA, MILENA A.
author_sort STACHELSKA, MILENA A.
collection PubMed
description The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic Y. enterocolitica in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the ail gene. Additionally, biochemical tests and RT-PCR showed that nine Y. enterocolitica isolates carried the ail-gene. Two Y. enterocolitica isolates of 1A biotype had the ail gene. The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the ail gene Y. enterocolitica in the material examined.
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spelling pubmed-72568032020-06-03 Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR STACHELSKA, MILENA A. Pol J Microbiol Microbiology The application of DNA-based methods enables to identify Yersinia enterocolitica carrying the ail-gene with a greater sensitivity compared to culture methods and biochemical tests used for detection of pathogenic Y. enterocolitica in animal and food samples. In this study, 100 samples of pig tonsils were examined, among which 17 were positive for the ail gene. Additionally, biochemical tests and RT-PCR showed that nine Y. enterocolitica isolates carried the ail-gene. Two Y. enterocolitica isolates of 1A biotype had the ail gene. The results demonstrated the usefulness of RT-PCR method applied for detection of potentially pathogenic, possessing the ail gene Y. enterocolitica in the material examined. Exeley Inc. 2018-06 2018-06-30 /pmc/articles/PMC7256803/ /pubmed/30015460 http://dx.doi.org/10.21307/pjm-2018-023 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology
STACHELSKA, MILENA A.
Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR
title Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR
title_full Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR
title_fullStr Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR
title_full_unstemmed Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR
title_short Identification of Pathogenicity of Yersinia enterocolitica in Pig Tonsils Using the Real-Time PCR
title_sort identification of pathogenicity of yersinia enterocolitica in pig tonsils using the real-time pcr
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256803/
https://www.ncbi.nlm.nih.gov/pubmed/30015460
http://dx.doi.org/10.21307/pjm-2018-023
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