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Aspergillus penicillioides Speg. Implicated in Keratomycosis

The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. M...

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Autores principales: MACHOWICZ-MATEJKO, EULALIA, FURMAŃCZYK, AGNIESZKA, ZALEWSKA, EWA DOROTA
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256836/
https://www.ncbi.nlm.nih.gov/pubmed/30550227
http://dx.doi.org/10.21307/pjm-2018-049
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author MACHOWICZ-MATEJKO, EULALIA
FURMAŃCZYK, AGNIESZKA
ZALEWSKA, EWA DOROTA
author_facet MACHOWICZ-MATEJKO, EULALIA
FURMAŃCZYK, AGNIESZKA
ZALEWSKA, EWA DOROTA
author_sort MACHOWICZ-MATEJKO, EULALIA
collection PubMed
description The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis.
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spelling pubmed-72568362020-06-03 Aspergillus penicillioides Speg. Implicated in Keratomycosis MACHOWICZ-MATEJKO, EULALIA FURMAŃCZYK, AGNIESZKA ZALEWSKA, EWA DOROTA Pol J Microbiol Microbiology The aim of the study was mycological examination of ulcerated corneal tissues from an ophthalmic patient. Tissue fragments were analyzed on potato-glucose agar (PDA) and maltose (MA) (Difco) media using standard laboratory techniques. Cultures were identified using classical and molecular methods. Macro- and microscopic colony morphology was characteristic of fungi from the genus Aspergillus (restricted growth series), most probably Aspergillus penicillioides Speg. Molecular analysis of the following rDNA regions: ITS1, ITS2, 5.8S, 28S rDNA, LSU and β-tubulin were carried out for the isolates studied. A high level of similarity was found between sequences from certain rDNA regions, i.e. ITS1-5.8S-ITS2 and LSU, what confirmed the classification of the isolates to the species A. penicillioides. The classification of our isolates to A. penicillioides species was confirmed also by the phylogenetic analysis. Exeley Inc. 2018-12 2018-12-10 /pmc/articles/PMC7256836/ /pubmed/30550227 http://dx.doi.org/10.21307/pjm-2018-049 Text en © 2018 Eulalia Machowicz-Matejko et al. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology
MACHOWICZ-MATEJKO, EULALIA
FURMAŃCZYK, AGNIESZKA
ZALEWSKA, EWA DOROTA
Aspergillus penicillioides Speg. Implicated in Keratomycosis
title Aspergillus penicillioides Speg. Implicated in Keratomycosis
title_full Aspergillus penicillioides Speg. Implicated in Keratomycosis
title_fullStr Aspergillus penicillioides Speg. Implicated in Keratomycosis
title_full_unstemmed Aspergillus penicillioides Speg. Implicated in Keratomycosis
title_short Aspergillus penicillioides Speg. Implicated in Keratomycosis
title_sort aspergillus penicillioides speg. implicated in keratomycosis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256836/
https://www.ncbi.nlm.nih.gov/pubmed/30550227
http://dx.doi.org/10.21307/pjm-2018-049
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