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Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni
The proteomes of outer membrane vesicles (OMVs) secreted by C. jejuni 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains – ∆dsbI and ∆htrA. OMVs production was significantly...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Exeley Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256860/ https://www.ncbi.nlm.nih.gov/pubmed/31250596 http://dx.doi.org/10.33073/pjm-2019-027 |
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author | GODLEWSKA, RENATA KLIM, JOANNA DĘBSKI, JANUSZ WYSZYŃSKA, AGNIESZKA ŁASICA, ANNA |
author_facet | GODLEWSKA, RENATA KLIM, JOANNA DĘBSKI, JANUSZ WYSZYŃSKA, AGNIESZKA ŁASICA, ANNA |
author_sort | GODLEWSKA, RENATA |
collection | PubMed |
description | The proteomes of outer membrane vesicles (OMVs) secreted by C. jejuni 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains – ∆dsbI and ∆htrA. OMVs production was significantly increased under the polymyxin B stress and remained unaltered in all other variants. Interestingly, the qualitative load of OMVs was constant regardless of the stress conditions or genetic background. However, certain proteins exhibited notable quantitative changes, ranging from 4-fold decrease to 10-fold increase. Up- or downregulated proteins (e.g. major outer membrane protein porA, iron ABC transporter, serine protease- htrA, 60 kDa chaperonin-groL, enolase) represented various cell compartments (cytoplasm, periplasm, and membrane) and exhibited various functions; nevertheless, one common group was noted that consisted of components of flagellar apparatus, i.e., FlaA/B, FlgC/E, which were mostly upregulated. Some of these proteins are the putative substrates of DsbI protein. Further investigation of the regulation of C. jejuni OMVs composition and their role in virulence will allow a better understanding of the infectious process of C. jejuni. |
format | Online Article Text |
id | pubmed-7256860 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Exeley Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72568602020-06-03 Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni GODLEWSKA, RENATA KLIM, JOANNA DĘBSKI, JANUSZ WYSZYŃSKA, AGNIESZKA ŁASICA, ANNA Pol J Microbiol Microbiology The proteomes of outer membrane vesicles (OMVs) secreted by C. jejuni 81–176 strain, which was exposed to oxygen or antibiotic stress (polymyxin B), were characterized. We also assessed the OMVs production and their content in two mutated strains – ∆dsbI and ∆htrA. OMVs production was significantly increased under the polymyxin B stress and remained unaltered in all other variants. Interestingly, the qualitative load of OMVs was constant regardless of the stress conditions or genetic background. However, certain proteins exhibited notable quantitative changes, ranging from 4-fold decrease to 10-fold increase. Up- or downregulated proteins (e.g. major outer membrane protein porA, iron ABC transporter, serine protease- htrA, 60 kDa chaperonin-groL, enolase) represented various cell compartments (cytoplasm, periplasm, and membrane) and exhibited various functions; nevertheless, one common group was noted that consisted of components of flagellar apparatus, i.e., FlaA/B, FlgC/E, which were mostly upregulated. Some of these proteins are the putative substrates of DsbI protein. Further investigation of the regulation of C. jejuni OMVs composition and their role in virulence will allow a better understanding of the infectious process of C. jejuni. Exeley Inc. 2019-06 2019-06-28 /pmc/articles/PMC7256860/ /pubmed/31250596 http://dx.doi.org/10.33073/pjm-2019-027 Text en © 2019 Renata Godlewska et al. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Microbiology GODLEWSKA, RENATA KLIM, JOANNA DĘBSKI, JANUSZ WYSZYŃSKA, AGNIESZKA ŁASICA, ANNA Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni |
title | Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni |
title_full | Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni |
title_fullStr | Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni |
title_full_unstemmed | Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni |
title_short | Influence of Environmental and Genetic Factors on Proteomic Profiling of Outer Membrane Vesicles from Campylobacter jejuni |
title_sort | influence of environmental and genetic factors on proteomic profiling of outer membrane vesicles from campylobacter jejuni |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256860/ https://www.ncbi.nlm.nih.gov/pubmed/31250596 http://dx.doi.org/10.33073/pjm-2019-027 |
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