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Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity

Paeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in...

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Autores principales: YANG, RUI-XIAN, ZHANG, SHAO-WEN, XUE, DONG, XUAN, JUN-HAO, ZHANG, YUAN-BO, PENG, BIAO-BIAO
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256872/
https://www.ncbi.nlm.nih.gov/pubmed/30550230
http://dx.doi.org/10.21307/pjm-2018-052
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author YANG, RUI-XIAN
ZHANG, SHAO-WEN
XUE, DONG
XUAN, JUN-HAO
ZHANG, YUAN-BO
PENG, BIAO-BIAO
author_facet YANG, RUI-XIAN
ZHANG, SHAO-WEN
XUE, DONG
XUAN, JUN-HAO
ZHANG, YUAN-BO
PENG, BIAO-BIAO
author_sort YANG, RUI-XIAN
collection PubMed
description Paeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in China. Subsequent characterization of 56 bacterial strains by 16S rDNA gene sequence analysis revealed that nine families and 13 different genera were represented. All the fungal strains were classed into six families and 12 genera based on ITS gene sequence. The biosynthetic potential of all the endophytes was further investigated by the detection of putative polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) genes. The PCR screens were successful in targeting thirteen bacterial PKS, five bacterial NRPS, ten fungal PKS and nine fungal NRPS gene fragments. Bioinformatic analysis of these detected endophyte gene fragments facilitated inference of the potential bioactivity of endophyte bioactive products, suggesting that the isolated endophytes are capable of producing a plethora of secondary metabolites. These results suggest that endophytes isolated from P. ostii had abundant population diversity and biosynthetic potential, which further proved that endophytes are valuable reservoirs of novel bioactive compounds.
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spelling pubmed-72568722020-06-03 Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity YANG, RUI-XIAN ZHANG, SHAO-WEN XUE, DONG XUAN, JUN-HAO ZHANG, YUAN-BO PENG, BIAO-BIAO Pol J Microbiol Microbiology Paeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in China. Subsequent characterization of 56 bacterial strains by 16S rDNA gene sequence analysis revealed that nine families and 13 different genera were represented. All the fungal strains were classed into six families and 12 genera based on ITS gene sequence. The biosynthetic potential of all the endophytes was further investigated by the detection of putative polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) genes. The PCR screens were successful in targeting thirteen bacterial PKS, five bacterial NRPS, ten fungal PKS and nine fungal NRPS gene fragments. Bioinformatic analysis of these detected endophyte gene fragments facilitated inference of the potential bioactivity of endophyte bioactive products, suggesting that the isolated endophytes are capable of producing a plethora of secondary metabolites. These results suggest that endophytes isolated from P. ostii had abundant population diversity and biosynthetic potential, which further proved that endophytes are valuable reservoirs of novel bioactive compounds. Exeley Inc. 2018-12 2018-12-10 /pmc/articles/PMC7256872/ /pubmed/30550230 http://dx.doi.org/10.21307/pjm-2018-052 Text en © 2018 Rui-xian Yang et al. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology
YANG, RUI-XIAN
ZHANG, SHAO-WEN
XUE, DONG
XUAN, JUN-HAO
ZHANG, YUAN-BO
PENG, BIAO-BIAO
Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity
title Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity
title_full Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity
title_fullStr Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity
title_full_unstemmed Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity
title_short Culturable Endophytes Diversity Isolated from Paeonia ostii and the Genetic Basis for Their Bioactivity
title_sort culturable endophytes diversity isolated from paeonia ostii and the genetic basis for their bioactivity
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256872/
https://www.ncbi.nlm.nih.gov/pubmed/30550230
http://dx.doi.org/10.21307/pjm-2018-052
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