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A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

[Image: see text] The cellulosome is a supramolecular multienzymatic protein complex that functions as a biological nanomachine of cellulosic biomass degradation. How the megadalton-size cellulosome adapts to a solid substrate is central to its mechanism of action and is also key for its efficient u...

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Autores principales: Eibinger, Manuel, Ganner, Thomas, Plank, Harald, Nidetzky, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256933/
https://www.ncbi.nlm.nih.gov/pubmed/32490190
http://dx.doi.org/10.1021/acscentsci.0c00050
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author Eibinger, Manuel
Ganner, Thomas
Plank, Harald
Nidetzky, Bernd
author_facet Eibinger, Manuel
Ganner, Thomas
Plank, Harald
Nidetzky, Bernd
author_sort Eibinger, Manuel
collection PubMed
description [Image: see text] The cellulosome is a supramolecular multienzymatic protein complex that functions as a biological nanomachine of cellulosic biomass degradation. How the megadalton-size cellulosome adapts to a solid substrate is central to its mechanism of action and is also key for its efficient use in bioconversion applications. We report time-lapse visualization of crystalline cellulose degradation by individual cellulosomes from Clostridium thermocellum by atomic force microscopy. Upon binding to cellulose, the cellulosomes switch to elongated, even filamentous shapes and morph these dynamically at below 1 min time scale according to requirements of the substrate surface under attack. Compared with noncomplexed cellulases that peel off material while sliding along crystalline cellulose surfaces, the cellulosomes remain bound locally for minutes and remove the material lying underneath. The consequent roughening up of the surface leads to an efficient deconstruction of cellulose nanocrystals both from the ends and through fissions within. Distinct modes of cellulose nanocrystal deconstruction by nature’s major cellulase systems are thus revealed.
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spelling pubmed-72569332020-06-01 A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose Eibinger, Manuel Ganner, Thomas Plank, Harald Nidetzky, Bernd ACS Cent Sci [Image: see text] The cellulosome is a supramolecular multienzymatic protein complex that functions as a biological nanomachine of cellulosic biomass degradation. How the megadalton-size cellulosome adapts to a solid substrate is central to its mechanism of action and is also key for its efficient use in bioconversion applications. We report time-lapse visualization of crystalline cellulose degradation by individual cellulosomes from Clostridium thermocellum by atomic force microscopy. Upon binding to cellulose, the cellulosomes switch to elongated, even filamentous shapes and morph these dynamically at below 1 min time scale according to requirements of the substrate surface under attack. Compared with noncomplexed cellulases that peel off material while sliding along crystalline cellulose surfaces, the cellulosomes remain bound locally for minutes and remove the material lying underneath. The consequent roughening up of the surface leads to an efficient deconstruction of cellulose nanocrystals both from the ends and through fissions within. Distinct modes of cellulose nanocrystal deconstruction by nature’s major cellulase systems are thus revealed. American Chemical Society 2020-05-06 2020-05-27 /pmc/articles/PMC7256933/ /pubmed/32490190 http://dx.doi.org/10.1021/acscentsci.0c00050 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Eibinger, Manuel
Ganner, Thomas
Plank, Harald
Nidetzky, Bernd
A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose
title A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose
title_full A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose
title_fullStr A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose
title_full_unstemmed A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose
title_short A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose
title_sort biological nanomachine at work: watching the cellulosome degrade crystalline cellulose
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7256933/
https://www.ncbi.nlm.nih.gov/pubmed/32490190
http://dx.doi.org/10.1021/acscentsci.0c00050
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