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PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells
AIMS: Toll-like receptor 4 (TLR4) and 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB3) are involved in the progress of inflammation and glucose metabolism. Here, we aimed to assess the relationship between TLR4 and PFKFB3 in liver cells. METHODS: We detected the expression of TLR4 and PF...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7257845/ https://www.ncbi.nlm.nih.gov/pubmed/32523673 http://dx.doi.org/10.1177/2042018820923474 |
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author | Lu, Yan Zhang, Lei Zhu, Ran Zhou, Huijuan Fan, Huaying Wang, Qiang |
author_facet | Lu, Yan Zhang, Lei Zhu, Ran Zhou, Huijuan Fan, Huaying Wang, Qiang |
author_sort | Lu, Yan |
collection | PubMed |
description | AIMS: Toll-like receptor 4 (TLR4) and 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB3) are involved in the progress of inflammation and glucose metabolism. Here, we aimed to assess the relationship between TLR4 and PFKFB3 in liver cells. METHODS: We detected the expression of TLR4 and PFKFB3 in both normal liver cell lines and liver cancer cell lines. Then, a small interfering RNA (siRNA) was used to knock down the expression of TLR4 and analyze the expression of PFKFB3 in the HL-7702 cell line. Further, following stimulation of the HL-7702 cell line with free fatty acids (FFA) or insulin, we observed the expression of TLR4 and PFKFB3, respectively. RESULTS: Knocking down siRNA-mediated TLR4 significantly reduced PFKFB3 expression at the mRNA and protein level. Furthermore, activating TLR4 with FFA dramatically increased PFKFB3 expression. Insulin increased the expression of TLR4 and PFKFB3, which could be inhibited by TLR siRNA. CONCLUSION: These findings suggest that PFKFB3 expression is regulated via the TLR4–PFKFB3 axis, which might be a bridge linking fat and glucose metabolism. |
format | Online Article Text |
id | pubmed-7257845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-72578452020-06-09 PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells Lu, Yan Zhang, Lei Zhu, Ran Zhou, Huijuan Fan, Huaying Wang, Qiang Ther Adv Endocrinol Metab Original Research AIMS: Toll-like receptor 4 (TLR4) and 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB3) are involved in the progress of inflammation and glucose metabolism. Here, we aimed to assess the relationship between TLR4 and PFKFB3 in liver cells. METHODS: We detected the expression of TLR4 and PFKFB3 in both normal liver cell lines and liver cancer cell lines. Then, a small interfering RNA (siRNA) was used to knock down the expression of TLR4 and analyze the expression of PFKFB3 in the HL-7702 cell line. Further, following stimulation of the HL-7702 cell line with free fatty acids (FFA) or insulin, we observed the expression of TLR4 and PFKFB3, respectively. RESULTS: Knocking down siRNA-mediated TLR4 significantly reduced PFKFB3 expression at the mRNA and protein level. Furthermore, activating TLR4 with FFA dramatically increased PFKFB3 expression. Insulin increased the expression of TLR4 and PFKFB3, which could be inhibited by TLR siRNA. CONCLUSION: These findings suggest that PFKFB3 expression is regulated via the TLR4–PFKFB3 axis, which might be a bridge linking fat and glucose metabolism. SAGE Publications 2020-05-27 /pmc/articles/PMC7257845/ /pubmed/32523673 http://dx.doi.org/10.1177/2042018820923474 Text en © The Author(s), 2020 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Lu, Yan Zhang, Lei Zhu, Ran Zhou, Huijuan Fan, Huaying Wang, Qiang PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells |
title | PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells |
title_full | PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells |
title_fullStr | PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells |
title_full_unstemmed | PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells |
title_short | PFKFB3, a key glucose metabolic enzyme regulated by pathogen recognition receptor TLR4 in liver cells |
title_sort | pfkfb3, a key glucose metabolic enzyme regulated by pathogen recognition receptor tlr4 in liver cells |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7257845/ https://www.ncbi.nlm.nih.gov/pubmed/32523673 http://dx.doi.org/10.1177/2042018820923474 |
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