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Gene editing in CHO cells to prevent proteolysis and enhance glycosylation: Production of HIV envelope proteins as vaccine immunogens

Several candidate HIV subunit vaccines based on recombinant envelope (Env) glycoproteins have been advanced into human clinical trials. To facilitate biopharmaceutical production, it is necessary to produce these in CHO (Chinese Hamster Ovary) cells, the cellular substrate used for the manufacturing...

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Detalles Bibliográficos
Autores principales:	Li, Sophia W., Wright, Meredith, Healey, John F., Hutchinson, Jennie M., O’Rourke, Sara, Mesa, Kathryn A., Lollar, Pete, Berman, Phillip W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7259603/ https://www.ncbi.nlm.nih.gov/pubmed/32470085 http://dx.doi.org/10.1371/journal.pone.0233866

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