Oriented immobilization of basic fibroblast growth factor: Bioengineered surface design for the expansion of human mesenchymal stromal cells

E. coli expressed recombinant basic fibroblast growth factor (bFGF) with histidine-tag (bFGF-His) was immobilized onto the surface of a glass plate modified with a Ni(II)-chelated alkanethiol monolayer. The immobilization is expected to take place through the coordination between Ni(II) and His-tag....

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Detalles Bibliográficos
Autores principales: Shakya, Ajay, Imado, Eiji, Nguyen, Phuong Kim, Matsuyama, Tamamo, Horimoto, Kotaro, Hirata, Isao, Kato, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260242/
https://www.ncbi.nlm.nih.gov/pubmed/32472000
http://dx.doi.org/10.1038/s41598-020-65572-2

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