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Condensin I is required for faithful meiosis in Drosophila males

The heteropentameric condensin complexes play vital roles in the formation and faithful segregation of mitotic chromosomes in eukaryotes. While the different contributions of the two common condensin complexes, condensin I and condensin II, to chromosome morphology and behavior in mitosis have been...

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Autores principales: Kleinschnitz, Kristina, Vießmann, Nina, Jordan, Mareike, Heidmann, Stefan K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260282/
https://www.ncbi.nlm.nih.gov/pubmed/32314039
http://dx.doi.org/10.1007/s00412-020-00733-w
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author Kleinschnitz, Kristina
Vießmann, Nina
Jordan, Mareike
Heidmann, Stefan K.
author_facet Kleinschnitz, Kristina
Vießmann, Nina
Jordan, Mareike
Heidmann, Stefan K.
author_sort Kleinschnitz, Kristina
collection PubMed
description The heteropentameric condensin complexes play vital roles in the formation and faithful segregation of mitotic chromosomes in eukaryotes. While the different contributions of the two common condensin complexes, condensin I and condensin II, to chromosome morphology and behavior in mitosis have been thoroughly investigated, much less is known about the specific roles of the two complexes during meiotic divisions. In Drosophila melanogaster, faithful mitotic divisions depend on functional condensin I, but not on condensin II. However, meiotic divisions in Drosophila males require functional condensin II subunits. The role of condensin I during male meiosis in Drosophila has been unresolved. Here, we show that condensin I-specific subunits localize to meiotic chromatin in both meiosis I and II during Drosophila spermatogenesis. Live cell imaging reveals defects during meiotic divisions after RNAi-mediated knockdown of condensin I-specific mRNAs. This phenotype correlates with reduced male fertility and an increase in nondisjunction events both in meiosis I and meiosis II. Consistently, a reduction in male fertility was also observed after proteasome-mediated degradation of the condensin I subunit Barren. Taken together, our results demonstrate an essential role of condensin I during male meiosis in Drosophila melanogaster. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00412-020-00733-w) contains supplementary material, which is available to authorized users.
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spelling pubmed-72602822020-06-08 Condensin I is required for faithful meiosis in Drosophila males Kleinschnitz, Kristina Vießmann, Nina Jordan, Mareike Heidmann, Stefan K. Chromosoma Original Article The heteropentameric condensin complexes play vital roles in the formation and faithful segregation of mitotic chromosomes in eukaryotes. While the different contributions of the two common condensin complexes, condensin I and condensin II, to chromosome morphology and behavior in mitosis have been thoroughly investigated, much less is known about the specific roles of the two complexes during meiotic divisions. In Drosophila melanogaster, faithful mitotic divisions depend on functional condensin I, but not on condensin II. However, meiotic divisions in Drosophila males require functional condensin II subunits. The role of condensin I during male meiosis in Drosophila has been unresolved. Here, we show that condensin I-specific subunits localize to meiotic chromatin in both meiosis I and II during Drosophila spermatogenesis. Live cell imaging reveals defects during meiotic divisions after RNAi-mediated knockdown of condensin I-specific mRNAs. This phenotype correlates with reduced male fertility and an increase in nondisjunction events both in meiosis I and meiosis II. Consistently, a reduction in male fertility was also observed after proteasome-mediated degradation of the condensin I subunit Barren. Taken together, our results demonstrate an essential role of condensin I during male meiosis in Drosophila melanogaster. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00412-020-00733-w) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2020-04-08 2020 /pmc/articles/PMC7260282/ /pubmed/32314039 http://dx.doi.org/10.1007/s00412-020-00733-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Kleinschnitz, Kristina
Vießmann, Nina
Jordan, Mareike
Heidmann, Stefan K.
Condensin I is required for faithful meiosis in Drosophila males
title Condensin I is required for faithful meiosis in Drosophila males
title_full Condensin I is required for faithful meiosis in Drosophila males
title_fullStr Condensin I is required for faithful meiosis in Drosophila males
title_full_unstemmed Condensin I is required for faithful meiosis in Drosophila males
title_short Condensin I is required for faithful meiosis in Drosophila males
title_sort condensin i is required for faithful meiosis in drosophila males
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260282/
https://www.ncbi.nlm.nih.gov/pubmed/32314039
http://dx.doi.org/10.1007/s00412-020-00733-w
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