Cargando…

Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy

Penaeus monodon is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in P. monodon through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from th...

Descripción completa

Detalles Bibliográficos
Autores principales: ABDUL HANNAN, MD., MAHBUBUR RAHMAN, MD., NURUNNABI MONDAL, MD., SUZAN CHANDRA, DEB, CHOWDHURY, GAZLIMA, TOFAZZAL ISLAM, MD.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Exeley Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260635/
https://www.ncbi.nlm.nih.gov/pubmed/31880887
http://dx.doi.org/10.33073/pjm-2019-042
_version_ 1783540359780892672
author ABDUL HANNAN, MD.
MAHBUBUR RAHMAN, MD.
NURUNNABI MONDAL, MD.
SUZAN CHANDRA, DEB
CHOWDHURY, GAZLIMA
TOFAZZAL ISLAM, MD.
author_facet ABDUL HANNAN, MD.
MAHBUBUR RAHMAN, MD.
NURUNNABI MONDAL, MD.
SUZAN CHANDRA, DEB
CHOWDHURY, GAZLIMA
TOFAZZAL ISLAM, MD.
author_sort ABDUL HANNAN, MD.
collection PubMed
description Penaeus monodon is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in P. monodon through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from the infected farm and the bacteria were isolated from the infected region of those samples. Based on phenotypic identification, several isolates were identified as Vibrio sp. 16S rRNA gene sequences of the selected isolates exhibited 100% homology with V. alginolyticus strain ATCC 17749. An in vivo infection challenge test was performed by immersion method with V. alginolyticus where these isolates caused high mortality in juvenile shrimp with prominent symptoms of hepatopancreatic necrosis. Antibiogram profile of the isolates was determined against eleven commercial antibiotic discs whereas the isolates were found resistant to multiple antibiotics. A total of twenty-one herbal extracts were screened where Emblica officinalis, Allium sativum, and Syzygium aromaticum strongly inhibited the growth of V. alginolyticus in in vitro conditions. In in vivo conditions, the ethyl acetate extracts of E. officinalis and A. sativum successfully controlled the vibriosis disease in shrimp at a dose of 10 mg/g feed. This is the first report on molecular identification and biocontrol of V. alginolyticus in shrimp in Bangladesh.
format Online
Article
Text
id pubmed-7260635
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Exeley Inc.
record_format MEDLINE/PubMed
spelling pubmed-72606352020-06-03 Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy ABDUL HANNAN, MD. MAHBUBUR RAHMAN, MD. NURUNNABI MONDAL, MD. SUZAN CHANDRA, DEB CHOWDHURY, GAZLIMA TOFAZZAL ISLAM, MD. Pol J Microbiol Microbiology Penaeus monodon is highly susceptible to vibriosis disease. Aims of the study were to identify the pathogen causing vibriosis in P. monodon through molecular techniques and develop a biocontrol method of the disease by application of herbal extracts. Shrimp samples were collected aseptically from the infected farm and the bacteria were isolated from the infected region of those samples. Based on phenotypic identification, several isolates were identified as Vibrio sp. 16S rRNA gene sequences of the selected isolates exhibited 100% homology with V. alginolyticus strain ATCC 17749. An in vivo infection challenge test was performed by immersion method with V. alginolyticus where these isolates caused high mortality in juvenile shrimp with prominent symptoms of hepatopancreatic necrosis. Antibiogram profile of the isolates was determined against eleven commercial antibiotic discs whereas the isolates were found resistant to multiple antibiotics. A total of twenty-one herbal extracts were screened where Emblica officinalis, Allium sativum, and Syzygium aromaticum strongly inhibited the growth of V. alginolyticus in in vitro conditions. In in vivo conditions, the ethyl acetate extracts of E. officinalis and A. sativum successfully controlled the vibriosis disease in shrimp at a dose of 10 mg/g feed. This is the first report on molecular identification and biocontrol of V. alginolyticus in shrimp in Bangladesh. Exeley Inc. 2019-12 2019-10-31 /pmc/articles/PMC7260635/ /pubmed/31880887 http://dx.doi.org/10.33073/pjm-2019-042 Text en © 2019 Marta Kłos et al. https://creativecommons.org/licenses/by-nc-nd/4.0/ https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Microbiology
ABDUL HANNAN, MD.
MAHBUBUR RAHMAN, MD.
NURUNNABI MONDAL, MD.
SUZAN CHANDRA, DEB
CHOWDHURY, GAZLIMA
TOFAZZAL ISLAM, MD.
Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy
title Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy
title_full Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy
title_fullStr Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy
title_full_unstemmed Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy
title_short Molecular Identification of Vibrio alginolyticus Causing Vibriosis in Shrimp and Its Herbal Remedy
title_sort molecular identification of vibrio alginolyticus causing vibriosis in shrimp and its herbal remedy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260635/
https://www.ncbi.nlm.nih.gov/pubmed/31880887
http://dx.doi.org/10.33073/pjm-2019-042
work_keys_str_mv AT abdulhannanmd molecularidentificationofvibrioalginolyticuscausingvibriosisinshrimpanditsherbalremedy
AT mahbuburrahmanmd molecularidentificationofvibrioalginolyticuscausingvibriosisinshrimpanditsherbalremedy
AT nurunnabimondalmd molecularidentificationofvibrioalginolyticuscausingvibriosisinshrimpanditsherbalremedy
AT suzanchandradeb molecularidentificationofvibrioalginolyticuscausingvibriosisinshrimpanditsherbalremedy
AT chowdhurygazlima molecularidentificationofvibrioalginolyticuscausingvibriosisinshrimpanditsherbalremedy
AT tofazzalislammd molecularidentificationofvibrioalginolyticuscausingvibriosisinshrimpanditsherbalremedy