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Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes
Plants produce a large variety of highly functionalized terpenoids. Functional groups such as partially unsaturated rings and carboxyl groups provide handles to use these compounds as feedstock for biobased commodity chemicals. For instance, methylperillate, a monoterpenoid found in Salvia dorisiana...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260718/ https://www.ncbi.nlm.nih.gov/pubmed/32090266 http://dx.doi.org/10.1093/jxb/eraa086 |
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author | Jongedijk, Esmer Müller, Sebastian van Dijk, Aalt D J Schijlen, Elio Champagne, Antoine Boutry, Marc Levisson, Mark van der Krol, Sander Bouwmeester, Harro Beekwilder, Jules |
author_facet | Jongedijk, Esmer Müller, Sebastian van Dijk, Aalt D J Schijlen, Elio Champagne, Antoine Boutry, Marc Levisson, Mark van der Krol, Sander Bouwmeester, Harro Beekwilder, Jules |
author_sort | Jongedijk, Esmer |
collection | PubMed |
description | Plants produce a large variety of highly functionalized terpenoids. Functional groups such as partially unsaturated rings and carboxyl groups provide handles to use these compounds as feedstock for biobased commodity chemicals. For instance, methylperillate, a monoterpenoid found in Salvia dorisiana, may be used for this purpose, as it carries both an unsaturated ring and a methylated carboxyl group. The biosynthetic pathway of methylperillate in plants is still unclear. In this work, we identified glandular trichomes from S. dorisiana as the location of biosynthesis and storage of methylperillate. mRNA from purified trichomes was used to identify four genes that can encode the pathway from geranyl diphosphate towards methylperillate. This pathway includes a (–)-limonene synthase (SdLS), a limonene 7-hydroxylase (SdL7H, CYP71A76), and a perillyl alcohol dehydrogenase (SdPOHDH). We also identified a terpene acid methyltransferase, perillic acid O-methyltransferase (SdPAOMT), with homology to salicylic acid OMTs. Transient expression in Nicotiana benthamiana of these four genes, in combination with a geranyl diphosphate synthase to boost precursor formation, resulted in production of methylperillate. This demonstrates the potential of these enzymes for metabolic engineering of a feedstock for biobased commodity chemicals. |
format | Online Article Text |
id | pubmed-7260718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-72607182020-06-03 Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes Jongedijk, Esmer Müller, Sebastian van Dijk, Aalt D J Schijlen, Elio Champagne, Antoine Boutry, Marc Levisson, Mark van der Krol, Sander Bouwmeester, Harro Beekwilder, Jules J Exp Bot Research Papers Plants produce a large variety of highly functionalized terpenoids. Functional groups such as partially unsaturated rings and carboxyl groups provide handles to use these compounds as feedstock for biobased commodity chemicals. For instance, methylperillate, a monoterpenoid found in Salvia dorisiana, may be used for this purpose, as it carries both an unsaturated ring and a methylated carboxyl group. The biosynthetic pathway of methylperillate in plants is still unclear. In this work, we identified glandular trichomes from S. dorisiana as the location of biosynthesis and storage of methylperillate. mRNA from purified trichomes was used to identify four genes that can encode the pathway from geranyl diphosphate towards methylperillate. This pathway includes a (–)-limonene synthase (SdLS), a limonene 7-hydroxylase (SdL7H, CYP71A76), and a perillyl alcohol dehydrogenase (SdPOHDH). We also identified a terpene acid methyltransferase, perillic acid O-methyltransferase (SdPAOMT), with homology to salicylic acid OMTs. Transient expression in Nicotiana benthamiana of these four genes, in combination with a geranyl diphosphate synthase to boost precursor formation, resulted in production of methylperillate. This demonstrates the potential of these enzymes for metabolic engineering of a feedstock for biobased commodity chemicals. Oxford University Press 2020-05-30 2020-02-24 /pmc/articles/PMC7260718/ /pubmed/32090266 http://dx.doi.org/10.1093/jxb/eraa086 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Jongedijk, Esmer Müller, Sebastian van Dijk, Aalt D J Schijlen, Elio Champagne, Antoine Boutry, Marc Levisson, Mark van der Krol, Sander Bouwmeester, Harro Beekwilder, Jules Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes |
title | Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes |
title_full | Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes |
title_fullStr | Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes |
title_full_unstemmed | Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes |
title_short | Novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from Salvia dorisiana trichomes |
title_sort | novel routes towards bioplastics from plants: elucidation of the methylperillate biosynthesis pathway from salvia dorisiana trichomes |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7260718/ https://www.ncbi.nlm.nih.gov/pubmed/32090266 http://dx.doi.org/10.1093/jxb/eraa086 |
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