Pre-concentration of microalga Euglena gracilis by alkalescent pH treatment and flocculation mechanism of Ca(3)(PO(4))(2), Mg(3)(PO(4))(2), and derivatives

BACKGROUND: Microalgae are widely be used in carbon sequestration, food supplements, natural pigments, polyunsaturated fatty acids, biofuel applications, and wastewater treatment. However, the difficulties incurred in algae cell separation and harvesting, and the exorbitant cost required to overcome these challenges, are the primary limitations to large-scale industrial application of microalgae technology. RESULTS: Herein, we explore the potential of inducing flocculation by adjusting the pH for pre-concentrating Euglena gracilis. Our results demonstrate that flocculation can be induced by increasing the medium pH to 8.5; however, most of the algae cells were broken by increasing the pH > 10. Magnesium phosphate, calcium phosphate, and their derivatives precipitation jointly led to flocculation, although calcium phosphate and its derivatives precipitation had a greater effect. CONCLUSIONS: This study demonstrates that pH treatment-induced flocculation is efficient and feasible for the pre-concentration of E. gracilis under a pilot-scale culture system. Moreover, it also maintained the microalgae cells’ integrity, chlorophyll production, and increased paramylon production. These findings provide a theoretical basis for reducing the cost of large-scale E. gracilis harvesting; as well as provide a reference for harvesting other microalgae.