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Characterization of Cas12a nucleases reveals diverse PAM profiles between closely-related orthologs

CRISPR-Cas systems comprise diverse adaptive immune systems in prokaryotes whose RNA-directed nucleases have been co-opted for various technologies. Recent efforts have focused on expanding the number of known CRISPR-Cas subtypes to identify nucleases with novel properties. However, the functional d...

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Detalles Bibliográficos
Autores principales:	Jacobsen, Thomas, Ttofali, Fani, Liao, Chunyu, Manchalu, Srinivas, Gray, Benjamin N, Beisel, Chase L
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261169/ https://www.ncbi.nlm.nih.gov/pubmed/32329776 http://dx.doi.org/10.1093/nar/gkaa272

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