Simultaneous measurement of biochemical phenotypes and gene expression in single cells

Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogenei...

Descripción completa

Detalles Bibliográficos
Autores principales: Richer, Amanda L, Riemondy, Kent A, Hardie, Lakotah, Hesselberth, Jay R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261187/
https://www.ncbi.nlm.nih.gov/pubmed/32286626
http://dx.doi.org/10.1093/nar/gkaa240
Descripción
Sumario:Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.

Ejemplares similares