Simultaneous measurement of biochemical phenotypes and gene expression in single cells

Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogenei...

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Autores principales: Richer, Amanda L, Riemondy, Kent A, Hardie, Lakotah, Hesselberth, Jay R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261187/
https://www.ncbi.nlm.nih.gov/pubmed/32286626
http://dx.doi.org/10.1093/nar/gkaa240
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author Richer, Amanda L
Riemondy, Kent A
Hardie, Lakotah
Hesselberth, Jay R
author_facet Richer, Amanda L
Riemondy, Kent A
Hardie, Lakotah
Hesselberth, Jay R
author_sort Richer, Amanda L
collection PubMed
description Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes.
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spelling pubmed-72611872020-06-03 Simultaneous measurement of biochemical phenotypes and gene expression in single cells Richer, Amanda L Riemondy, Kent A Hardie, Lakotah Hesselberth, Jay R Nucleic Acids Res Methods Online Methods to measure heterogeneity among cells are rapidly transforming our understanding of biology but are currently limited to molecular abundance measurements. We developed an approach to simultaneously measure biochemical activities and mRNA abundance in single cells to understand the heterogeneity of DNA repair activities across thousands of human lymphocytes, identifying known and novel cell-type-specific DNA repair phenotypes. Oxford University Press 2020-06-04 2020-04-14 /pmc/articles/PMC7261187/ /pubmed/32286626 http://dx.doi.org/10.1093/nar/gkaa240 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Richer, Amanda L
Riemondy, Kent A
Hardie, Lakotah
Hesselberth, Jay R
Simultaneous measurement of biochemical phenotypes and gene expression in single cells
title Simultaneous measurement of biochemical phenotypes and gene expression in single cells
title_full Simultaneous measurement of biochemical phenotypes and gene expression in single cells
title_fullStr Simultaneous measurement of biochemical phenotypes and gene expression in single cells
title_full_unstemmed Simultaneous measurement of biochemical phenotypes and gene expression in single cells
title_short Simultaneous measurement of biochemical phenotypes and gene expression in single cells
title_sort simultaneous measurement of biochemical phenotypes and gene expression in single cells
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261187/
https://www.ncbi.nlm.nih.gov/pubmed/32286626
http://dx.doi.org/10.1093/nar/gkaa240
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