The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches

The hirudin-like factors 3 (HLF3) and 4 (HLF4) belong to a new class of leech-derived factors and are present in specimens of the three European medicinal leeches, Hirudo medicinalis, Hirudo verbana, and Hirudo orientalis, respectively. Here we describe the functional analysis of natural and synthet...

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Autores principales: Müller, Christian, Lukas, Phil, Sponholz, Dana, Hildebrandt, Jan-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Genetics, Evolution, and Phylogeny - Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261268/
https://www.ncbi.nlm.nih.gov/pubmed/32363441
http://dx.doi.org/10.1007/s00436-020-06697-1
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author Müller, Christian
Lukas, Phil
Sponholz, Dana
Hildebrandt, Jan-Peter
author_facet Müller, Christian
Lukas, Phil
Sponholz, Dana
Hildebrandt, Jan-Peter
author_sort Müller, Christian
collection PubMed
description The hirudin-like factors 3 (HLF3) and 4 (HLF4) belong to a new class of leech-derived factors and are present in specimens of the three European medicinal leeches, Hirudo medicinalis, Hirudo verbana, and Hirudo orientalis, respectively. Here we describe the functional analysis of natural and synthetic variants of HLF3 and HLF4. Whereas the natural variants display only very low or no detectable anti-coagulatory activities, modifications within the N-termini in combination with an exchange of the central globular domain have the potency to greatly enhance the inhibitory effects of respective HLF3 and HLF4 variants on blood coagulation. Our results support previous observations on the crucial importance of all parts (both the N- and C-termini as well as the central globular domains) of hirudin and HLF molecules for thrombin inhibition.
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spelling pubmed-72612682020-06-10 The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches Müller, Christian Lukas, Phil Sponholz, Dana Hildebrandt, Jan-Peter Parasitol Res Genetics, Evolution, and Phylogeny - Original Paper The hirudin-like factors 3 (HLF3) and 4 (HLF4) belong to a new class of leech-derived factors and are present in specimens of the three European medicinal leeches, Hirudo medicinalis, Hirudo verbana, and Hirudo orientalis, respectively. Here we describe the functional analysis of natural and synthetic variants of HLF3 and HLF4. Whereas the natural variants display only very low or no detectable anti-coagulatory activities, modifications within the N-termini in combination with an exchange of the central globular domain have the potency to greatly enhance the inhibitory effects of respective HLF3 and HLF4 variants on blood coagulation. Our results support previous observations on the crucial importance of all parts (both the N- and C-termini as well as the central globular domains) of hirudin and HLF molecules for thrombin inhibition. Springer Berlin Heidelberg 2020-05-04 2020 /pmc/articles/PMC7261268/ /pubmed/32363441 http://dx.doi.org/10.1007/s00436-020-06697-1 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Genetics, Evolution, and Phylogeny - Original Paper
Müller, Christian
Lukas, Phil
Sponholz, Dana
Hildebrandt, Jan-Peter
The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches
title The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches
title_full The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches
title_fullStr The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches
title_full_unstemmed The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches
title_short The hirudin-like factors HLF3 and HLF4—hidden hirudins of European medicinal leeches
title_sort hirudin-like factors hlf3 and hlf4—hidden hirudins of european medicinal leeches
topic Genetics, Evolution, and Phylogeny - Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7261268/
https://www.ncbi.nlm.nih.gov/pubmed/32363441
http://dx.doi.org/10.1007/s00436-020-06697-1
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